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随着杀虫剂在仓库中的广泛应用,仓库害虫的抗药性种类普遍增多,抗药性程度日益发展。要研究抗性,首先要掌握培育抗性与测定抗性的方法。本文根据世界粮农组织(FAO)统一采用的方法,结合我国具体情况加以补充,提供读者参考。 一、抗药性的培育 1.药粉法 这种方法适用于赤拟谷盗,在30℃的温度和70％的相对湿度的条件下,用含有杀虫剂的全麦粒饲养。这里介绍赤拟谷盗对DDT抗性培育的一种方法。饲料的配制是以足够的苯溶解适量的DDT,使它完全浸泡全麦粉,并经过充分拌和,然后使苯在通风橱中蒸发。每代选择使用的饲料和药剂浓度应当时配制,干燥的全麦粉在饲养前再经过拌和。选择从5、10、20ppm三组浓度开始,每组在有全麦粉200克的玻璃瓶中分别放入成虫70头,5周后将成虫筛出弃
With the wide application of pesticides in warehouses, the varieties of drug resistance in storage pests generally increase, and the degree of resistance is increasing day by day. To study resistance, we must first master the methods of cultivating resistance and measuring resistance. This article is based on the uniform approach adopted by the World Food Program (FAO) and supplemented by the specific conditions of our country to provide readers reference. First, the resistance of the cultivation of 1. Powder method This method is suitable for red tarry Pirates, at a temperature of 30 ℃ and 70% relative humidity conditions, with whole-crop insecticide feeding. Here is a brief description of the method of cultivating DDT resistance by Trichosanthes. The feed was prepared by dissolving the right amount of DDT in enough benzene to completely soak the whole wheat flour and mixing it well before the benzene was allowed to evaporate in a fume hood. The feed and drug concentration selected for each generation should be prepared at that time, and the dried whole wheat flour is mixed before being raised. Select the concentration from the beginning of the three groups of 5,10,20 ppm, each group with 200 grams of whole wheat flour into the glass bottles were 70 adult head, 5 weeks after the adult sieve was discarded