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观察了细锯脂鲤脂鳍和背鳍形态和骨骼发育,在此基础上比较了脂鳍、背鳍原基和背鳍共3个组织的转录组,发现细锯脂鲤背鳍原基28 DAH(Day After Hatch,DAH)出现,30 DAH仔鱼背鳍鳍条已出现分化,35 DAH背鳍鳍条已硬骨化,脂鳍在32 DAH开始出现。背鳍原基、背鳍和脂鳍这3个组织共同表达基因有14 115条,分别占背鳍原基转录组的84.01%、背鳍的83.19%、脂鳍的84.14%,提示脂鳍发育的主要调控机制与背鳍发育较为相似。相同基因表达水平差异方面,相比脂鳍,背鳍原基和背鳍之间的基因表达模式要更为相似。发现有些与鳍发育相关的基因在背鳍和脂鳍转录组中存在差异。这些研究结果为进一步深入了解脂鳍发育奠定了基础。
The morphological and skeletal development of adipose fins and dorsal fins were observed. Based on the results, the transcriptome of adipose fins, dorsal fin dorsal fin and dorsal fin were compared. It was found that DAI (Day After Hatch, DAH) appeared, 30 DAH larvae had differentiated fin rays, 35 DAH dorsal fin rays had become sclerotic, and fat fins began to appear at 32 DAH. There were 14,115 genes coexpressed in the three tissues of dorsal fin, dorsal fin and adipose fin, accounting for 84.01%, 83.19%, and 84.14% respectively of the dorsal fin primordial transcriptome, indicating the main regulatory mechanism of the fat fin development And dorsal fin development is more similar. In terms of differences in the same gene expression, the pattern of gene expression is more similar than that between the fat fins, the dorsal fin primordia and the dorsal fin. Some genes related to fin development were found to differ in the dorsal fin and fin transcriptome. These findings provide the basis for further understanding of the development of lipid fins.