论文部分内容阅读
目的:研究孕期铅暴露对子代大鼠学习记忆功能的影响及其可能的分子机制。方法:通过饮用0.02%醋酸铅水溶液建立大鼠孕期染铅模型,正常饮水为对照组,21 d新生鼠为研究对象,各组12只。检测血铅,利用Morris水迷宫分析系统检测大鼠的学习记忆能力,通过高尔基(Golgi)染色方法观察海马CA1及DG区神经元树突棘的形态和数量的变化。结果:铅暴露组P21新生鼠血铅为31.75±4.83μg/dl,显著高于对照组P21新生鼠(0.96±0.17μg/dl,P<0.01);铅暴露组子代大鼠在定位航行实验中的上台潜伏期较对照组显著延长(P<0.05),在空间探索实验中的目标象限停留时间也较对照组显著降低(P<0.01);CA1区锥体细胞和DG区颗粒细胞的树突棘以蘑菇型和细长型树突棘为主,铅暴露后树突棘主要以粗短型为主。铅暴露组子代CA1和DG去树突棘的密度显著低于对照组(P<0.01)。结论:孕期铅暴露对子代学习记忆功能的影响可能与海马区神经元树突棘的形态变化和密度降低有关。
Objective: To study the influence of lead exposure during pregnancy on the learning and memory function of offspring rats and its possible molecular mechanism. Methods: The model of lead exposure during pregnancy was established by drinking 0.02% aqueous solution of lead acetate. Normal drinking water was used as the control group. Twelve days old neonatal rats were used as study subjects, with 12 rats in each group. Blood lead levels were measured. Morris water maze analysis system was used to detect the learning and memory abilities of rats. The morphology and number of dendritic spines in hippocampal CA1 and DG neurons were observed by Golgi staining. Results: The level of blood lead in lead exposed P21 group was 31.75 ± 4.83μg / dl, which was significantly higher than that in control group P21 newborn mice (0.96 ± 0.17μg / dl, P <0.01). In lead exposure group, (P <0.05), and the target quadrant residence time in space exploration experiment was significantly lower than that in control group (P <0.01). The dendrites of pyramidal cell and DG in CA1 area Spine to mushroom-type and elongated dendritic spine-based, dendritic spine after lead exposure mainly to short and short-based. The densities of dendritic spines of CA1 and DG were significantly lower in the lead exposed group than in the control group (P <0.01). Conclusion: The effects of lead exposure during pregnancy on the learning and memory abilities of offspring may be related to the morphological changes and densities of dendritic spines in hippocampal neurons.