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目的探讨跨代染镉对子代大鼠学习记忆的影响。方法将36只8周大的SD大鼠随机分为4组:饲料中不添加镉组(1组);饲料中按1 mg/kg加入镉组(2组);饲料中按5 mg/kg加入镉组(3组);饲料中按25 mg/kg加入镉组(4组)。雌雄分开饲养,一月后按照雌雄2∶1的比例合笼,产下的幼鼠同亲代大鼠镉暴露方式和剂量继续接触镉,待幼鼠成长至8周,Morris水迷宫检测大鼠学习记忆能力;石墨炉原子吸收仪测定亲代和子代大鼠肝、肾、脑中镉含量和大脑内锌含量。Western bolt测定海马、纹状体、皮质内SYP-1的表达。结果 2组和3组第3天和第4天水定向航行时间延长,差异有统计学意义(P<0.05)。各组穿越平台与穿越有效区域的次数差异无统计学意义。随着染镉剂量的增加,亲代、子代新生和子代8周大鼠大脑、肝脏和肾镉含量逐渐增高,脑内锌含量逐渐降低。各组亲代大鼠肝脏、肾脏和脑镉的含量与子代新生大鼠中镉含量均呈正相关。2、3、4组与1组比较,海马、纹状体和皮质内SYP-1的表达明显降低,差异有统计学意义(P<0.05)。结论镉对大鼠学习记忆有一定影响。
Objective To investigate the effects of cross-generation cadmium exposure on learning and memory in offspring rats. Methods Thirty-six 8-week-old SD rats were randomly divided into 4 groups: no cadmium group (1 group), 1 mg / kg dietary cadmium group (2 groups) and 5 mg / kg Add cadmium group (3 groups); feed by 25 mg / kg cadmium group (4 groups). The male and female were kept separately. After January, they were caged in the ratio of 2: 1 for male and 2 female. The neonatal rats were exposed to cadmium exposure and the dose of cadmium. The rats were allowed to grow for up to 8 weeks. Morris water maze test was used to study rats Memory capacity; Graphite furnace atomic absorption spectrometry was used to determine the contents of cadmium and zinc in the liver, kidney and brain of the offspring and offspring rats. Western bolt determination of SYP-1 expression in the hippocampus, striatum, cortex. Results The water navigation time of groups 2 and 3 was longer on the 3rd and 4th days, with statistical significance (P <0.05). There was no significant difference in the number of crossing the platform between the groups and the effective area. With the increase of cadmium dose, the content of cadmium in the brain, liver and kidney of the offspring, progeny, and offspring of neonatal and progeny rats gradually increased and the content of zinc in the brain decreased gradually. The liver, kidney and brain cadmium contents of the parental rats in each group were positively correlated with the cadmium content in offspring neonatal rats. The expression of SYP-1 in hippocampus, striatum and cortex were significantly decreased in groups 2, 3, 4 compared with those in group 1, with significant difference (P <0.05). Conclusion Cadmium has certain influence on learning and memory in rats.