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目的:观察芪芍胶囊对糖尿病肾病大鼠模型血液生化指标及对肾组织促血管生成素1(Ang-1)表达的影响,探讨芪芍胶囊对糖尿病肾病大鼠的肾保护作用和可能机制。方法:将60只大鼠随机选取10只为正常对照组,其余50只采用高糖高脂饲料并腹腔注射链脲佐菌素(STZ)的方法复制糖尿病肾病大鼠模型,造模成功后大鼠随机分为模型对照组、芪芍胶囊(450mg/kg、900mg/kg、1800mg/kg)组、厄贝沙坦组,各组按相应剂量灌胃,在给药的第8周末检测大鼠24小时尿蛋白定量(UTP),腹主动脉取血,测定血清总蛋白(TP)、白蛋白(ALB)、总胆固醇(TC)、甘油三酯(TG)、尿素氮(BUN)、肌酐(Scr)水平;电镜下观察肾脏病理形态学变化;免疫组化及Real-time PCR法检测各组大鼠肾组织Ang-1的表达情况。结果:与模型对照组比较,各治疗组UTP明显下降,TC、TG明显降低,芪芍胶囊(450mg/kg、900mg/kg、1800mg/kg)组TC下降显著;TP、ALB均明显升高。与厄贝沙坦组比较,芪芍胶囊(900mg/kg、1800mg/kg)组TC、TG均明显降低,厄贝沙坦组与芪芍胶囊(450mg/kg、900mg/kg、1800mg/kg)组UTP及TP、ALB比较差距无统计学意义。与正常对照组比较,各治疗组及模型对照组BUN、Scr均未见明显变化。与模型对照组比较,各治疗组Ang-1mRNA表达量均明显降低;厄贝沙坦组与芪芍胶囊(450mg/kg、900mg/kg、1800mg/kg)组相比差异无统计学意义。结论:芪芍胶囊对糖尿病肾病大鼠的肾保护作用可能与其能够下调肾组织中Ang-1的表达有关。
OBJECTIVE: To observe the effects of Qishao capsule on blood biochemical parameters and the expression of Ang-1 in diabetic nephropathy rats and to explore the renal protective effect of Qishao capsule on diabetic nephropathy rats and its possible mechanism. Methods: Ten rats from 60 rats were randomly selected as the normal control group. The remaining 50 rabbits were randomly divided into high glucose and high fat diet and intraperitoneal injection of streptozotocin (STZ) to replicate the model of diabetic nephropathy rats. The rats were randomly divided into model control group, Qishao capsule (450mg / kg, 900mg / kg, 1800mg / kg) group, irbesartan group, each group were given the corresponding dose of gavage, 24-hour urinary protein (UTP) and abdominal aorta blood were collected for determination of serum total protein (TP), albumin (ALB), total cholesterol (TC), triglyceride (TG), blood urea nitrogen (BUN) Scr). The pathological changes of kidney were observed under electron microscope. The expression of Ang-1 in kidney of each group was detected by immunohistochemistry and Real-time PCR. Results: Compared with the model control group, the levels of UTP, TC and TG in each treatment group decreased significantly. The TC levels in the rats treated with Qishao Capsule (450mg / kg, 900mg / kg and 1800mg / kg) decreased significantly. Compared with irbesartan group, the TC and TG in the Qishao capsule (900mg / kg, 1800mg / kg) group were significantly lower than those in the irbesartan group and the irbesartan group (450mg / kg, 900mg / kg, 1800mg / kg) Group UTP and TP, ALB difference was not statistically significant. Compared with the normal control group, no significant changes were found in BUN and Scr in each treatment group and model control group. Compared with the model control group, the expression of Ang-1mRNA in each treatment group was significantly decreased; there was no significant difference between irbesartan group and Qishao capsule (450mg / kg, 900mg / kg, 1800mg / kg) Conclusion: The renal protective effect of Qishao capsule on diabetic nephropathy rats may be related to its ability to down-regulate the expression of Ang-1 in renal tissues.