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以5种氨基甲酸酯类农药涕灭威(ALD)、残杀威(BAY)、呋喃丹(CAR)、灭多威(MET)和抗蚜威(PIR)为研究对象，应用均匀设计射线法设计五元混合物体系共6条射线(U1、U2、…、U6)，应用基于发光菌青海弧菌Q67的微板毒性分析法(MTA)系统地考察了5种农药及其混合物的毒性，以浓度加和(CA)为参考模型分析混合物毒性相互作用(协同或拮抗作用)。结果表明，Logit和Weibull函数能较好地拟合5种氨基甲酸酯农药及其混合物对发光菌Q67的浓度-效应数据(R > 0.99, RMSE < 0.032)；以EC50的负对数值pEC50为毒性指标，5种农药的毒性顺序为BAY (pEC50 =2.87) > CAR (pEC50 =2.67) > ALD (pEC50 =2.00) > MET (pEC50 =1.99) > PIR(pEC50 =1.79)；依据CA，五元氨基甲酸酯类农药的6条混合物射线中，有2条呈加和作用，4条呈拮抗作用，其中U2和U4在整条浓度-效应曲线上呈现了明显的拮抗作用，而U3和U6的弱拮抗作用分别发生在混合物浓度的中高浓度区和中低浓度区；五元氨基甲酸酯类农药混合物的毒性与组分灭多威(MET)的浓度比呈良好的负相关关系(R= -0.9238)，且线性模型对混合物毒性具有良好的预测能力。
Five kinds of carbamate pesticides aldicarb (ALD), carnitine (BAY), carbofuran (CAR), methomyl (MET) and pirimicarb (PIR) were selected as the research objects. (U1, U2, ..., U6) of the five-component mixture system, the toxicity of five kinds of pesticides and their mixtures was systematically investigated using the microplate toxicity assay (MTA) based on the light-emitting strain Qinghai Vibrio Q67, Mixture (CA) was used as a reference model to analyze mixtures for toxic interactions (synergistic or antagonistic). The results showed that Logit and Weibull function could well fit concentration-effect data of five carbamate pesticides and their mixtures to Q67 of luminescent bacteria (R> 0.99, RMSE <0.032) The pEC50 is a toxicological indicator and the toxicity order of the five pesticides is BAY (pEC50 = 2.87) & gt; CAR (pEC50 = 2.67) & gt; ALD (pEC50 = 2.00) & gt; MET ; According to CA, two of five mixtures of five carbamate pesticides were additive and four were antagonistic, of which U2 and U4 showed obvious antagonism on the whole concentration-response curve , While the weak antagonism of U3 and U6 occurred in the middle and high concentrations of the mixture and in the middle and low concentrations of the mixture, respectively. The toxicity of the five-component carbamate pesticide mixture was negatively correlated with the concentration of the component methomyl (MET) (R = -0.9238), and the linear model has good predictive ability on the toxicity of the mixture.