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用bolbosum技术检验来自中国春×农林61的F1代产生的52个双单倍体系(DH)的麦谷蛋白高分子量(HMW)亚基等位基因和农艺性状间的关系。同时,研究这些DH的赤霉素不敏感半矮等位基因的效应。中国春编码麦谷蛋白HMW亚基的等位基因是Glu-Alc和Glu-Dla,农林61则是Glu-Alb和Glu-Dlf.DH系按两种HMW亚基位点重组可分为四组,仅Glu-Alc·Glu-Dlf较其余三种基因型植株较高和抽穗时间较长.农林61携带有GA不敏感等位基因Gai/Rht2,而中国春对GA敏感。按Gai/Rht2位点又可将这些DH系分为两组。凡是Gal/Rht2的基因型植株都显著较gai/rhtZ植株矮,籽粒产量则显著提高。Glu—Alc和Glu—Dlf促进株高的结合效应几乎被Gai/Rht2降低株高的效应所抵消。这些结果说明麦谷蛋白基因位点的选择显著影响普通小麦某些农艺性状的表达。
The relationship between the HMW subunit alleles and agronomic traits of 52 double haplogroups (DH) generated from F1 generation of Chinese Spring × Agriculture and Forestry 61 was tested by bolbosum technique. At the same time, the effect of these DH gibberellin-insensitive semidwarf alleles was studied. The alleles of the HMW subunit of Chinese spring-coded glutenin are Glu-Alc and Glu-Dla, and Gluten-Glu and Glu-Dlf are the dominant species in agriculture and forestry. DH lines can be divided into four groups according to the recombination of two subunit HMW subunits. Only Glu-Alc · Glu-Dlf is higher than the other three genotype plants and the heading time is longer. Agriculture and forestry 61 carry GA insensitive allele Gai / Rht2, while Chinese spring is sensitive to GA. These DH lines can be divided into two groups by Gai / Rht2 sites. All genotypes of Gal / Rht2 plants were significantly shorter than gai / rhtZ plants, grain yield was significantly increased. The combined effect of Glu-Alc and Glu-Dlf on plant height was almost offset by the effect of Gai / Rht2 on plant height reduction. These results indicate that the selection of glutenin loci significantly affects the expression of certain agronomic traits in common wheat.