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目的：分离和鉴别沙林和梭曼等含 Ｐ Ｆ键神经性毒剂光学异构体。方法：１９ Ｆ Ｎ Ｍ Ｒ 手性诱导位移。结果和结论：对沙林和梭曼等１１ 个含 Ｐ Ｆ 键的神经性毒剂的１９ Ｆ Ｎ Ｍ Ｒ 与结构关系进行了研究。提出了一种测定含 Ｐ Ｆ 键神经性毒剂光学异构体的方便而有效的方法。本方法基于在氘代苯中用三（３三氟甲基羟基亚甲基ｄ樟脑）铕［ Ｅｕ（ｔｆｃ）３］作为手性位移试剂，使含 Ｐ Ｆ 键神经性毒剂对映异构体的重叠１９ Ｆ Ｎ Ｍ Ｒ 信号完全达到分离。毒剂与血浆在 ３７℃孵育一定时间后，根据光学异构体被血液中存在的磷酰磷酸酯酶降解的速度不同而产生１９ Ｆ Ｎ Ｍ Ｒ 信号相对强度的差别，以达到鉴别各个异构体。研究表明， Ｐ（＋ ）异构体的１９ Ｆ Ｎ Ｍ Ｒ 诱导位移信号总是出现在 Ｐ（）异构体的低磁场方向。这个规律有助于鉴别这类毒剂的光学异构体。
OBJECTIVE: To separate and identify optical isomers of neurotoxins containing PF bonds such as sarin and soman. Methods: 19 FN M R chiral induced displacement. RESULTS AND CONCLUSION: The relationship between the 19 FN M R and the structure of eleven PF containing neurotoxic agents such as sarin and soman was studied. A convenient and effective method for the determination of optical isomers of P-F-containing neurotoxic agents was proposed. The method is based on the use of tris (3-trifluoromethylhydroxymethylene-d-camphor) europium [Eu (tfc) 3] as a chiral shift reagent in deuterated benzene so that the P-F neurotoxic agent pair Overlapped enantiomeric 19 F N NM signals completely resolved. Differences in the relative intensities of 19 FN M R signals occur depending on the rate of degradation of optical isomers by phosphorylphosphatase present in the blood after incubation of the agent with plasma at 37 ° C for a period of time to identify each isoforms body. The results show that the 19 FN M R induced displacement signal of P (+) is always observed in the low magnetic field of P () isomer. This rule helps identify the optical isomers of this type of agent.