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目的:探讨正丁基-β-D-吡喃果糖苷诱导肝癌Bel-7402细胞凋亡的作用机制。方法:采用四甲基偶氮唑蓝(MTT)比色法,检测正丁基-β-D-吡喃果糖苷对Bel-7402细胞的增殖抑制效应;将6.25,12.5,25mg·mL-1的正丁基-β-D-吡喃果糖苷作用于Bel-7402细胞24h和48h,采用流式细胞术(FCM)测定细胞周期及凋亡率;应用凝胶成像分析仪检测DNALadder。结果:正丁基-β-D-吡喃果糖苷在体外对Bel-7402细胞有增殖抑制效应,在正丁基-β-D-吡喃果糖苷作用下,Bel-7402细胞出现显著的细胞凋亡征象;流式细胞术检测结果显示,不同浓度的正丁基-β-D-吡喃果糖苷与Bel-7402细胞作用24,48h可明显抑制Bel-7402细胞存活,25mg·mL-1的正丁基-β-D-吡喃果糖苷抑制率达到42.39%;凝胶成像分析仪检测到典型的DNA阶梯状条带。结论:正丁基-β-D-吡喃果糖苷有诱导Bel-7402细胞凋亡的作用,且随着浓度的升高和作用时间的延长,其诱导细胞凋亡作用增强。
Objective: To investigate the mechanism of apoptosis induced by n-butyl-β-D-fructopyranoside in Bel-7402 hepatocellular carcinoma cells. Methods: MTT assay was used to detect the inhibitory effect of n-butyl-β-D-glucopyranoside on Bel-7402 cells. Six doses of 6.25, 12.5 and 25 mg · mL- N-butyl-β-D-glucopyranoside on Bel-7402 cells for 24h and 48h. The cell cycle and apoptosis rate were determined by flow cytometry (FCM). DNALadder was detected by gel imaging analyzer. Results: The inhibitory effect of n-butyl-β-D-glucopyranoside on Bel-7402 cells was inhibited in vitro. Under the action of n-butyl-β-D-fructopyranoside, The results of flow cytometry showed that the effects of different concentrations of n-butyl-β-D-glucopyranoside on Bel-7402 cells at 24 and 48 h could significantly inhibit the survival of Bel-7402 cells and 25 mg · mL -1 Of n-butyl-β-D-fructopyranoside inhibition rate of 42.39%; gel imaging analyzer detected a typical ladder DNA bands. CONCLUSION: n-Butyl-β-D-fructopyranoside can induce the apoptosis of Bel-7402 cells. With the increase of concentration and duration of action, the induction of apoptosis is enhanced.