【摘 要】
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目的 探讨繁殖和鉴定补体C3基因敲除小鼠的实验方法.方法 将所引进的补体C3基因敲除杂合子小鼠(C3+/-)进行饲养并繁殖,其子代出现三种基因型的小鼠,即纯合子C3-/-、杂合子C3+
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目的 探讨繁殖和鉴定补体C3基因敲除小鼠的实验方法.方法 将所引进的补体C3基因敲除杂合子小鼠(C3+/-)进行饲养并繁殖,其子代出现三种基因型的小鼠,即纯合子C3-/-、杂合子C3+/-、野生型C3+/+,采用ELISA与PCR相结合对子代小鼠基因型进行鉴定.结果 繁育出135只子代小鼠,经鉴定,C3+/+、C3+/-、C3-/-各为38只、68只、29只,经x2检验,子代小鼠分离比例符合孟德尔遗传规律.结论 正确的饲养繁殖以及子代鉴定是从杂合子小鼠中获得补体C3基因敲除小鼠的有效途径.
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