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应用巢式聚合酶链反应（NestedPCR）对74例HBVM阳性的孕妇外周静脉血及其新生儿脐血进行了HBVDNA检测。结果显示双抗原（HBsAg和HBeAg）阳性组HBVDNA检出率母血为100％（8／8），脐血为87．5％（7／8）。单抗原（HBsAg）阳性组HBVDNA检出率母血为26.92％（14／52），脐血为19．23％（10／52）。抗体（抗-HBc、抗-HBs、抗-HBe一项至三项）阳性组HBVDNA检出率母血为14．29％（2／14），职血为7．14％（1／14）。
Nested polymerase chain reaction (NestedPCR) in 74 cases of HBVM-positive pregnant women with peripheral venous blood and neonatal cord blood HBVDNA detection. The results showed that the detection rate of HBVDNA was 100% (8/8) in maternal blood and 87.5% (7/8) in umbilical cord blood. The detection rate of HBVDNA in HBsAg positive group was 26.92% (14/52) and that in cord blood was 19.23% (10/52). The detection rate of HBVDNA was 14.29% (2/14) in maternal blood and 7.14% (1/14) in serum in the positive group (anti-HBc, anti-HBs, anti-HBe one to three) .