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目的研究亚低温对易卒中型肾血管性高血压大鼠（ＲＨＲＳＰ）复制的大脑中动脉闭塞（ＭＣＡＯ）模型的梗死灶大小及对ＢＣＬ－２蛋白表达的影响。方法用环型银夹以双肾双夹法复制ＲＨＲＳＰ，改良Ｌｏｎｇａ’ｓ线栓法制成ＭＣＡＯ缺血模型，冰袋法诱导亚低温，ＴＴＣ染色测定梗死灶体积，免疫组化法、图象识别系统计算ＢＣＬ－２蛋白表达强度。结果１亚低温能明显缩小梗死灶体积；２亚低温组ＢＣＬ－２蛋白表达明显强于正常体温组。结论亚低温增强缺血后基因ＢＣＬ－２的表达可能是它具有缺血脑保护作用机制之一。
Objective To investigate the effects of mild hypothermia on the infarct size and the expression of BCL-2 protein in middle cerebral artery occlusion (MCAO) model of rats with stroke-induced renovascular hypertension (RHRSP). Methods RHRSP was duplicated with ring-shaped silver clip with twin-kidney method, MCAO model with modified Longa’s thread method, and infarct size with immunohistochemistry and image recognition system BCL-2 protein expression intensity was calculated. Results 1 mild hypothermia can significantly reduce infarct volume; 2 mild hypothermia group BCL-2 protein expression was significantly stronger than the normal body temperature group. CONCLUSION: Mild hypothermia enhances the expression of BCL-2 gene after ischemia may be one of the mechanisms of its protective effects on cerebral ischemia.