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以台湾原产中华结缕草（Ｚｏｙｓｉａｓｉｎｉｃａ）、沟叶结缕草（Ｚ．ｍａｔｒｅｌｌａ），山东原产中华结缕草、日本结缕草（Ｚ．ｊａｐｏｎｉｃａ），日本原产日本结缕草和细叶结缕草（Ｚ．ｔｅｎｕｉｆｏｉａ，包含一部分从人工草坪采集的样品）共２０２个样品为材料，检验其酯酶同工酶谱；结果发现了２６条酯酶带，构成了１３０个酶谱类型，各酶带出现频率随草种和原产地而异，以各酶带出现的频率，利用尤克利氏距离公式算出各族群的距离，发现中华结缕草和沟叶结缕草间的距离最近，合成一群集，细叶结缕草和日本结缕草距离最远，各成一群集。以上结果表明，酯酶同工酶不但有助于结缕草亲缘关系及基因多样化的研究，而且因酶谱类型繁多，雷同机会较少，可用作品种（系）鉴定的依据。
To Taiwan native Zoysiasinica (Zoysiasinica), Zoysia matrenica (Z. matrella), Shandong native Zoysia japonica, Zoysia japonica (Z.japonica), Japanese native Zoysia japonica and fine A total of 202 samples of Z. tenuifoia (including some samples collected from artificial turf) were used as materials to test the esterase isozyme pattern. As a result, 26 esterase bands were found, constituting 130 zymogram types , The frequency of each enzyme band varies with the grass species and the country of origin. The frequency of occurrence of each enzyme band was calculated using the Euclidean distance formula and the distance between each ethnic group was calculated. The distance between Zoysia sinica and Zoysia matrella was found closest , The synthesis of a cluster, Zoysia japonica and Zoysia japonica farthest distance, each into a cluster. The above results show that esterase isozyme not only contributes to the genetic relationship and gene diversity of Zoysia japonica, but also can be used as the basis for the identification of varieties (lines) due to the wide variety of zymograms and few similar opportunities.