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目的:研究尿液CMV-DNA联合血清CMV-Ig M检测对婴儿巨细胞感染的诊断价值。方法:选取2012年8月到2014年8月期间我院疑似巨细胞感染的婴幼儿70例,应用化学发光法检测血清CMV-Ig M,采用实时荧光定量PCR法(FQ-PCR)来检测尿液CMV-DNA,并比较经抗病毒治疗前和治疗后检测结果的变化。结果:单独检测尿CMV-DNA的阳性率为62.8%(44/70),单独检测血清CMV-Ig M的阳性率为55.7%(39/70),两者比较差异无统计学意义(P>0.05);两者联合检测的阳性率为77.1%(54/70),与两种方法单独检测比较差异具有统计学意义(P<0.05);经抗病毒治疗以后两种检测结果均显著降低,与治疗前比较差异具有统计学意义(P<0.05)。结论:尿液CMV-DNA联合血清CMV-Ig M检测能显著提高婴儿巨细胞感染的阳性率。
Objective: To investigate the diagnostic value of urine CMV-DNA combined with serum CMV-Ig M in diagnosis of infant’s cytomegalovirus infection. Methods: Seventy infants suspected to have cytomegalovirus infection in our hospital from August 2012 to August 2014 were enrolled in this study. Serum CMV-Ig M was detected by chemiluminescence assay and urine was detected by real-time fluorescence quantitative PCR (FQ-PCR) Liquid CMV-DNA, and compared before and after treatment with anti-virus test results. Results: The positive rate of CMV-DNA in urine alone was 62.8% (44/70), and the positive rate of CMV-Ig M in serum alone was 55.7% (39/70), with no significant difference between the two groups (P> 0.05). The positive rate of combined detection of the two methods was 77.1% (54/70), which was significantly different from that of the two methods (P <0.05). After the antiviral treatment, the two test results were significantly reduced, Compared with the pre-treatment difference was statistically significant (P <0.05). Conclusion: The detection of urine CMV-DNA combined with serum CMV-Ig M can significantly improve the positive rate of infant’s cytomegalovirus infection.