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目的:探讨白藜芦醇(Res)诱导宫颈癌HeLa细胞凋亡的作用及可能的机制。方法:MTT法检测不同浓度(0、12.5、25、50、100、200、300、400、600μmol/L)Res处理24、48、72h对HeLa细胞的抑制率;流式细胞仪采用AnnexinV和PI双染检测HeLa细胞的凋亡率。荧光显微镜观测细胞的形态学改变。分光光度法检测Caspase-3活性。结果:Res在一定浓度范围内,以浓度和时间依赖的方式抑制宫颈癌HeLa细胞的生长(P<0.05)。以0、200、300μmol/LRes处理细胞48小时,HeLa细胞早期凋亡率分别为2.2%、7.1%、6.23%,晚期凋亡率为7.7%、16.04%、15.43%。荧光显微镜下细胞呈现典型的凋亡性改变。200μmol/LRes处理8h后,Caspase-3活性增加,24h达高峰,后逐渐下降,经50、100、200μmol/LRes处理24h后,Caspase-3活性与对照组相比,分别增加了1.92倍、2.51倍、4.53倍(P<0.05)。结论:白藜芦醇通过诱导Caspase-3活性增加以时间和浓度依赖的方式抑制宫颈癌HeLa细胞增殖,诱导细胞凋亡。
Objective: To investigate the effect and possible mechanism of resveratrol on apoptosis of cervical cancer HeLa cells. Methods: The inhibitory rates of HeLa cells treated with different concentrations (0, 12.5, 25, 50, 100, 200, 300, 400 and 600 μmol / L) of Res for 24,48,72 h were determined by MTT assay. Annexin V and PI Double staining was used to detect the apoptosis rate of HeLa cells. Fluorescence microscopy was used to observe the morphological changes of cells. Spectrophotometric determination of Caspase-3 activity. Results Res inhibited the growth of cervical cancer HeLa cells in a concentration - and time - dependent manner (P <0.05). The apoptosis rate of HeLa cells was 2.2%, 7.1% and 6.23%, respectively. The apoptosis rate of late HeLa cells was 7.7%, 16.04% and 15.43% respectively at 0, 200 and 300μmol / Lres for 48 hours. Cells under fluorescence microscope showed typical apoptotic changes. After treated with 200μmol / LRes for 8h, Caspase-3 activity increased and peaked at 24h, then decreased gradually. After treated with 50, 100 and 200μmol / Lres for 24 h, Caspase-3 activity increased by 1.92-fold and 2.51 Times, 4.53 times (P <0.05). CONCLUSION: Resveratrol can inhibit the proliferation and induce the apoptosis of HeLa cells in a time-and-concentration-dependent manner by inducing the increase of Caspase-3 activity.