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背景:乙醇中毒可致神经系统广泛、严重的损害,并可降低自然杀伤细胞应答而使细胞免疫发生变化。但有关乙醇中毒性免疫损伤和脑损伤关系的研究较少。目的:探讨慢性乙醇中毒患者血清白细胞介素-6(Interleukin-6,IL-6)和肿瘤坏死因子α(Tumornecrosisfactoralpha,TNF-α)水平变化及其与乙醇中毒性脑萎缩的关系。设计:对照实验研究。地点和对象:地点为遵义医学院附属第一医院,对象为1999-02/2001-07门诊和住院的32例慢性乙醇中毒患者,均为男性,年龄24～64岁。发病年龄24～50岁,病程2个月～6年。30例对照系健康体检者,均为男性,年龄30～60岁。干预:用双抗体夹心酶联免疫吸附试验测定血清中IL-6和TNF-α水平,并结合头颅CT扫描进行分析。主要观察指标:以脑室指数、Huckman值、侧脑室体部指数及三脑室宽度判定脑萎缩程度。根据试剂盒的校正曲线计算血清中相应的IL-6和TNF-α浓度。结果:①慢性乙醇中毒组脑萎缩发生率为50%。②慢性乙醇中毒组血清IL-6和TNF-α水平犤分别为(286.31±104.79)ng/L,(413.34±66.87)ng/L犦显著高于健康对照组犤分别为(205.43±48.67)ng/L,(261.36±51.48)ng/L犦(P<0.001)。③慢性乙醇中毒有脑萎缩者血清IL-6和TNF-α水平犤分别为(343.75±99.59)ng/L,(449.38±55.79)ng/L犦高于无脑萎?
Background: Ethanol intoxication can cause extensive and severe damage to the nervous system and can reduce cellular immune responses in response to natural killer cells. However, there are few researches on the relationship between alcohol-induced immune damage and brain injury. Objective: To investigate the changes of serum levels of Interleukin-6 (IL-6) and tumor necrosis factor α (TNF-α) in patients with chronic alcoholism and their relationship with alcohol-induced brain atrophy. Design: Controlled experimental study. Location and Subjects: The first affiliated hospital of Zunyi Medical College, 32 outpatients and hospitalized patients with chronic alcoholism from February 1999 to July 2001 were male and 24 to 64 years old. Age of onset of 24 to 50 years old, duration of 2 months to 6 years. 30 cases of control health examination, all men, aged 30 to 60 years. Intervention: Serum levels of IL-6 and TNF-α were measured by double antibody sandwich enzyme-linked immunosorbent assay (ELISA) combined with cranial CT scan. MAIN OUTCOME MEASURES: Brain atrophy was judged by ventricular index, Huckman’s value, lateral ventricular mass index and third ventricle width. The corresponding concentrations of IL-6 and TNF-α in serum were calculated based on the calibration curve of the kit. Results: ① The incidence of cerebral atrophy in chronic alcoholism group was 50%. ② The levels of serum IL-6 and TNF-α in patients with chronic alcoholism were (286.31 ± 104.79) ng / L and (413.34 ± 66.87) ng / L, respectively, significantly higher than those in healthy controls (205.43 ± 48.67) ng /L, (261.36 ± 51.48) ng / L 犦 (P <0.001). ③ The levels of IL-6 and TNF-α in patients with chronic alcoholism and brain atrophy were (343.75 ± 99.59) ng / L and (449.38 ± 55.79) ng / L, respectively, higher than those without brain atrophy.