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目的 探讨ＴＮＦＲｐ５５和ＴＮＦＲｐ７５在各种肿瘤组织中的表达与新型ｒｈＴＮＦ对肿瘤细胞生长的抑制作用的关系。方法应用免疫组织化学染色法，观察１２０例肿瘤组织和１０株体外培养的肿瘤细胞中，ＴＮＦＲｐ５５和ＴＮＦＲｐ７５的表达及分布。同时应用结晶紫摄入法，观察ｎｒｈＴＮＦ对肿瘤细胞生长的抑制。结果 在肿瘤组织和肿瘤细胞中，ＴＮＦＲｐ５５的阳性表达率分别为８２．５％和９９％，ＴＭＦＲｐ７５分别为 ８４．２％和９８．８％，不同类型肿瘤间的表达无明显差异（Ｐ＞０．０５）．ｎｒｈＴＮＦ可抑制ＳＷ４８０、ＳＧＣ７９０１、８９１０ＳＭＭＣ７７２１和ＢＥＬ７４０２这５株肿瘤细胞生长，但是不能抑制另外５株肿瘤细胞Ａ５４９、ＰＬＡ８０１、Ｈｅｌａ、Ｅｃａ１０９和ＧＲＣ－ｌ的生长。结论ＴＮＦＲ广泛存在于各种肿瘤细胞上，ｎｒｈＴＮＦ抑制肿瘤细胞生长的程度与ＴＮＦＲｐ５５的表达率无相关性。
Objective To investigate the relationship between the expression of TNFRp55 and TNFRp75 in various tumor tissues and the inhibitory effect of rhTNF on tumor cell growth. Methods Immunohistochemical staining was used to observe the expression and distribution of TNFRp55 and TNFRp75 in 120 tumor tissues and 10 in vitro cultured tumor cells. At the same time, crystal violet intake method was used to observe the inhibition of tumor growth by nrhTNF. Results The positive expression rates of TNFRp55 in tumor tissues and tumor cells were 82.5% and 99%, respectively, and those of TMFRp75 were 84.2% and 98.8% respectively. There was no significant difference between different types of tumors (P> 0 .05). nrhTNF inhibited the growth of 5 tumor cells, SW480, SGC7901, 8910SMMC7721 and BEL7402, but did not inhibit the growth of the other 5 tumor cells, A549, PLA801, Hela, Eca109 and GRC-1. Conclusion TNFR exists in a wide range of tumor cells. The degree of tumor growth inhibition by nrhTNF is not correlated with the expression of TNFRp55.