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辣根过氧化物酶(Horseradish Peroxidase HRP)被广泛用于神经束路的追踪,HRP法同免疫细胞化学法相结合可确定投射神经元的递质性质。TMB法是显示HRP的最敏感的方法。但TMB反应产物在pH3.0~4.0
Horseradish Peroxidase (HRP) is widely used in the tracing of neural tracts. The combination of HRP and immunocytochemistry can determine the neurotransmitter properties of projecting neurons. The TMB method is the most sensitive method to show HRP. However, the TMB reaction product has a pH of 3.0 to 4.0