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目的和方法：采用ＡＢＣ免疫组织化学法结合图象分析，观察大鼠脑组织神经肽Ｙ、亮氨酸脑啡肽、强啡肽Ａ１１３ 在长期（ 共７ 周）大强度（速度由１５ ｍ／ｍｉｎ 递增至３５ ｍ／ｍｉｎ、运动时间为２０ ～２５ ｍｉｎ／ｄ） 的运动下的变化。结果：安静状态下在丘脑室旁核（ＰＶ） 、下丘脑背内侧核（ＤＭ） 、下丘脑腹内侧核（ＶＭＨ）等核团ＮＰＹ 无显著性变化；在此基础上的末次急性运动结束后３ ｈ ＮＰＹ 变化尤为明显。安静状态下大鼠尾壳核ＬＥＮＫ 下降；而末次急性运动后大鼠下丘脑ＬＥＮＫ 被迅速激活而升高。该强度运动能激活下丘脑ＤＹＮＡ１１３ ，尤以运动结束后３０ ｍｉｎ 最为明显。结论： ＮＰＹ、ＬＥＮＫ、ＤＹＮＡ１１３ 在该强度运动下大鼠不同脑区呈现不同变化趋势
PURPOSE AND METHODS: ABC immunohistochemistry combined with image analysis was used to observe the neuropeptide Y, leucine enkephalin, and dynorphin A113 in the long-term (total 7 weeks) m / min increased to 35 m / min, exercise time is 20 ~ 25 min / d) changes in exercise. Results: There was no significant change in NPY in nucleus paraventricular nucleus (PV), hypothalamic dorsal medial nucleus (DM) and hypothalamic ventromedial nucleus (VMH) in quiet state. On the basis of this, after the last acute exercise 3 h NPY changes are particularly evident. L-ENK decreased in the caudate putamen nucleus in a quiet state; while LENK in the hypothalamus of rats was rapidly activated after the last acute exercise. This intensity exercise can activate the hypothalamus DYNA1 13, especially 30 min after the end of the most obvious exercise. Conclusion: NPY, L ENK, DYNA1 13 at different intensities in different brain regions show different trends