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小麦种子经30kRγ射线照射后,再从M_1株取未成熟胚培养,用再生株当代(M_2R_1)的死苗率、不育株率、结实率及株高变化所反映的损伤程度与对照株(R_1)比较,无明显差异;M_3R_2代的变异率也与对照R_2相仿.5天龄幼胚用0.5kR照射处理后的再生株M_1R_1的损伤程度与对照相仿;M_2R_2的变异率随不同基因型而有增减,但总的变异率仍有显著提高.未成熟胚来源的愈伤组织经1kR照射处理的再生株C_1r_1的损伤加重,但变异率却提高3倍,有益变异增多.以上3种诱变处理,以愈伤组织照射处理结果较好,但照射量宜下降到0.5—1.0kR,幼胚照射效果次之.
Wheat seeds were irradiated with 30kR γ-rays, and then taken from M_1 immature embryos. The degree of seedling injury, the rate of sterile plants, the seed setting rate and plant height of regeneration plants were compared with that of the control plants ( R_1) were similar to those of the control (R_2). The damage degree of the M_1R_1 regenerated strain (M_1R_1) treated with 0.5kR irradiation was similar to that of the control, while the mutation rate of M_2R_2 varied with different genotypes But there was still a significant increase in the total mutation rate.Myrotic embryo-derived callus injury by 1kR irradiated C_1r_1 strain increased, but the mutation rate increased 3-fold, the beneficial variation increased more than three kinds of induced Change treatment, the callus irradiation treatment results are better, but the amount of radiation should be reduced to 0.5-1.0kR, followed by immature embryo irradiation.