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将起始浓度不同的单菌和混合菌(Ba.sp06+Str.v13)分别与铜绿微囊藻休眠体及底泥共包埋,在梯度温度下进行24 d休眠体复苏实验.结果表明,温度低于10℃时,菌株Str.v13的生长增殖能力显著强于菌株Ba.sp06,各菌组休眠体复苏率最高为0.02%;在温度为15和20℃条件下,Ba.sp06与Str.v13两菌株浓度比例(以下简称“菌比”)为1∶1时混合菌组休眠体复苏率(15℃时为15.5%,20℃时为23.5%)与对照组无显著差异(15℃时为15%,20℃时为22.6%),但显著低于Ba.sp06单菌组(34.5%);菌比2∶1时Ba.sp06菌对Str.v13菌具抑制效应,混合菌组复苏率(15℃时为31%,20℃时为35%)显著高于对照组,而与Ba.sp06单菌组无显著差异;菌比为1∶2时Str.v13菌对Ba.sp06菌具抑制效应,混合菌组休眠体复苏率(15℃时为6%,20℃时为6.5%)均显著低于菌比为1∶1、2∶1时及Ba.sp06单菌组,但高于Str.v13单菌组(3%).同时,各复苏阶段对复苏率的贡献主要集中在启动复苏的第12~18 d.实验表明,底泥表层铜绿微囊藻休眠体复苏不仅受温度影响(>10℃),且复苏率受底栖菌Ba.sp06与Str.v13的起始浓度影响,Ba.sp06菌的起始浓度高于Str.v13菌时促进休眠体复苏,低于Str.v13菌时复苏受到抑制.因此,可以在铜绿微囊藻休眠体复苏前期通过调节Ba.sp06与Str.v13菌比来调控休眠体复苏率,为从源头防控微囊藻水华暴发提供理论依据.
Bacillus licheniformis dormancy and sediment were co-embedded respectively with single bacteria and mixed bacteria (Ba.sp06 + Str.v13) with different initial concentrations, and the dormancy resuscitation experiments were carried out for 24 days at the gradient temperature.The results showed that, When the temperature was lower than 10 ℃, the growth and proliferation ability of strain Str.v13 was significantly stronger than that of strain Ba.sp06, and the highest rate of dormancy recovery was 0.02% in each strain. Under the conditions of 15 and 20 ℃, Ba.sp06 and Str The recovery rate of dormant body in mixed bacteria group (15.5% at 15 ℃ and 23.5% at 20 ℃) was not significantly different from that in control group when the ratio of the two strains of strain .v13 (hereinafter referred to as “bacteria ratio”) was 1: 1 15% at 15 ℃ and 22.6% at 20 ℃), but significantly lower than that of the Ba.sp06 single-bacteria group (34.5%). When the ratio of bacteria to bacteria was 2:1, the bacterium Str. The recovery rate of bacteria (31% at 15 ℃ and 35% at 20 ℃) was significantly higher than that of the control group, but not significantly different from that of the Ba.sp06 single bacteria group. When the bacteria ratio was 1: 2, .sp06, the rate of dormancy recovery (6% at 15 ℃ and 6.5% at 20 ℃) in the mixed bacteria group was significantly lower than that of the single strain at 1: 1, 2:1 and Ba.sp06 Group, but higher than Str.v13 single bacteria group (3%) .At the same time, the recovery phase of the recovery phase mainly concentrated in the contribution Dynamic recovery of the first 12 ~ 18 d. Experiments show that sediment surface layer Microcystis aeruginosa dormancy resuscitation not only affected by temperature (> 10 ℃), and the recovery rate by benthic bacteria Ba.sp06 and Str.v13 initial concentration , The initial concentration of Ba.sp06 was higher than that of Str.v13 to promote the resuscitation of resting body, and the recovery was inhibited when the strain was lower than Str.v13.At the same time, Compared with Str.v13 bacteria to regulate dormancy recovery rate, to provide a theoretical basis for the prevention and control of Microcystis bloom.