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目的 探讨检测前列腺癌(PC)患者外周血DD3mRNA在诊断和治疗监测中的意义。方法 用巢式逆转录聚合酶链反应(nRT PCR)检测44例不同分期PC患者和30例良性前列腺增生(BPH)患者及18名健康男性志愿者外周血中DD3mRNA,并进行对比分析。结果 BPH患者和健康男性志愿者外周血中DD3mRNA均阴性;未治疗PC组外周血DD3mRNA100%阳性(11 /11),而经内分泌治疗PC组阳性率30. 3% ( 10 /33 ),两组差异有统计学意义(P<0. 01 )。结论 外周血DD3mRNA是PC诊断和内分泌治疗监测的良好指标,有望成为PC特异性肿瘤标志物。
Objective To investigate the significance of detection of DD3 mRNA in peripheral blood of patients with prostate cancer (PC) in diagnosis and treatment. Methods The expression of DD3 mRNA in peripheral blood of 44 patients with different stages of PC, 30 benign prostatic hyperplasia (BPH) and 18 healthy volunteers was detected by nested reverse transcriptase polymerase chain reaction (nRT PCR) and compared. Results DD3 mRNA in peripheral blood of BPH patients and healthy male volunteers was negative. DD3 mRNA in peripheral blood of 100 U / ml PC group was 100% positive (11/11), while the positive rate of endocrine PC group was 30.3% (10/33) The difference was statistically significant (P <0.01). Conclusion Peripheral blood DD3mRNA is a good indicator of PC diagnosis and endocrine therapy monitoring and is expected to become a PC-specific tumor marker.