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以感病苹果品种‘嘎啦’为材料,采用喷雾接种方法,研究内生放线菌A-1诱导苹果对炭疽叶枯病的抗性,结果表明菌株A-1预处理后再接种病菌0101,叶片病斑数和病情指数均显著降低,防效达70.42%。对叶片中丙二醛(MDA)含量和防御相关酶活性动态变化进行测定,结果显示,接种病菌0101的处理,MDA含量快速上升,最大增幅为202.90%,而喷施菌株A-1的处理,MDA含量变化较小,最高增幅仅为23.74%;同时,菌株A-1显著提高了叶片中氧化物酶(POD)、过氧化氢酶(CAT)、超氧化物歧化酶(SOD)、多酚氧化酶(PPO)和苯丙氨酸解氨酶(PAL)的活性,且上述酶活性均高于接种病菌0101的处理。这表明菌株A-1通过提高‘嘎啦’苹果叶片中防御酶活性来减少活性氧的积累,降低膜脂过氧化产物MDA的形成,从而诱导寄主对炭疽叶枯病的抗性。
The susceptible apple cultivar ’Gala’ was used as material to study the resistance of endophytic actinomycete A-1 induced to anthracnose blight by spray inoculation method. The results showed that strain A-1 pretreated with bacterium 0101 , Leaf spot number and disease index were significantly reduced, the control effect of 70.42%. The dynamic changes of malondialdehyde (MDA) content and defense-related enzyme activity in leaves were measured. The results showed that the MDA content of the inoculated bacteria 0101 increased rapidly with the maximum increase of 202.90% The content of MDA changed little, the highest increase was only 23.74%. At the same time, strain A-1 significantly increased the content of POD, CAT, SOD, (PPO) and phenylalanine ammonia lyase (PAL) activity, and the above enzyme activity were higher than inoculation of bacteria 0101 treatment. This indicates that strain A-1 can reduce the accumulation of reactive oxygen species and decrease the formation of MDA, a membrane lipid peroxidation product, by increasing the defense enzyme activity in ’Gala apple’ leaves, thus inducing host resistance to anthracnose blight.