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目的:研究Fibulin-3在子宫骶韧带组织中的表达以及与脱垂程度之间的关系。方法:应用实时定量RT-PCR和Western blot测定子宫脱垂组(POP组)46例和正常对照组28例子宫骶韧带组织中Fibulin-3 mRNA及蛋白的相对表达量。结果:两组中Fib-ulin-3均有表达,POP组骶韧带中Fibulin-3 mRNA及蛋白表达均降低,POP组Fibulin-3mRNA表达水平为0.68±0.38低于对照组的0.96±0.44(P<0.05);POP组Fibulin-3蛋白表达水平为0.76±0.48低于对照组的1.14±0.52(P<0.05),且随脱垂程度的增加表达降低(P<0.05)。结论:Fibulin-3的表达与盆腔脏器脱垂的发生相关。
Objective: To study the relationship between the expression of Fibulin-3 in uterosacral ligaments and the degree of prolapse. Methods: The relative expression of Fibulin-3 mRNA and protein in uterosacral ligaments of 46 cases of uterine prolapse group (POP group) and 28 cases of normal control group were determined by real-time quantitative RT-PCR and Western blot. Results: Fib-ulin-3 was expressed in both groups. The expression of Fibulin-3 mRNA and protein in the sacral ligament of POP group was decreased. The expression level of Fibulin-3 mRNA in POP group was 0.68 ± 0.38, lower than that of the control group (0.96 ± 0.44, P <0.05). The expression level of Fibulin-3 in POP group was lower than that of control group (0.76 ± 0.48 vs 1.14 ± 0.52, P <0.05), and decreased with the degree of prolapse (P <0.05). Conclusion: The expression of Fibulin-3 correlates with pelvic organ prolapse.