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目的 :观察长期饥饿应激对肠神经系统神经递质的影响。方法 :以饥饿为应激原 ,采用肌间神经丛铺片技术 ,酶组织化学及免疫细胞化学法观察应激大鼠胃窦肌间神经丛内Ach、SP、NOS、SS、ENK及CCK神经变化。结果 :( 1)长期饥饿应激后大鼠胃电节律异常指数及慢波频率变异系数均显著增高。 ( 2 )大鼠胃窦肌间神经丛内Ach、SP神经含量明显减少 ;NOS、SS、ENK及CCK含量明显增加。结论 :( 1)长期饥饿应激能引起严重的胃电节律失常 ;( 2 )饥饿应激后大鼠胃电节律失常 ,胃窦肌间神经丛内Ach、SP神经分布异常 ,递质含量减少 ;NOS、SS、ENK、CCK神经含量异常增加。
Objective: To observe the effect of long-term starvation stress on the neurotransmitter in the enteric nervous system. Methods: Starvation was used as the stress source. The myelinated nerve fibers were collected from the myenteric plexus of rats with stress, and the myogenic distribution of Ach, SP, NOS, SS, ENK and CCK were detected by enzyme histochemistry and immunocytochemistry Variety. Results: (1) After long-term starvation stress, the anomaly index of gastric electrical rhythm and the coefficient of variation of slow wave frequency were significantly increased. (2) The contents of Ach and SP neurons in the myenteric plexus of the rat antral significantly decreased, and the content of NOS, SS, ENK and CCK increased significantly. Conclusion: (1) Long-term starvation stress can cause severe electrogastrogram; (2) After gastric starvation, gastric antrum dysfunction, abnormal distribution of Ach and SP nerve in gastric myenteric plexus, decrease of neurotransmitter content ; NOS, SS, ENK, CCK abnormal increase in nerve content.