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用免疫组化染色方法，观察了P物质受体在外周对伤害性刺激信息的介导作用。于福尔马林注入双侧后肢足底前10min，将不同浓度（10-4，10-5和10－6mol／L）的SP受体特异性拮抗剂L668，169注入一侧足底，另一侧注入生理盐水。结果：10-4mol／L的L668，169明显抑制了该侧脊髓背角浅层（Ⅰ、Ⅱ层）c-fos基因的表达，而对深层影响不大；注入浓度为10－5mol／L和10－6mol／L的L668，169对背角深、浅层的作用均不明显。另外，将SP受体激动剂Sar－SP（10-4mol／L）或福尔马林分别注入不同动物的一侧后肢足底，另一侧注射等量的生理盐水，均可引起激动剂或福尔马林注入侧脊髓背角深、浅层的FOS阳性神经元数量的增多。上述结果提示，SP在外周具有致痛作用，该作用至少部分是通过SP与SP受体结合而参与介导伤害性信息向脊髓传递。
Immunohistochemical staining was used to observe the effect of substance P receptors on nociceptive stimuli in the periphery. 10 min before injection of formalin into the hindlimbs of the hindlimbs, SP receptor-specific antagonists L668 and 169 at different concentrations (10-4, 10-5 and 10-6 mol / L) One side of the injection of saline. Results: L-668,169 (10-4 mol / L) significantly inhibited the expression of c-fos gene in the superficial layers of spinal dorsal horn (Ⅰ, Ⅱ) The effect of 10-6mol / L L668,169 on the dorsal horn was not obvious. In addition, the SP receptor agonist Sar-SP (10-4mol / L) or formalin were injected into the hindlimb of different animals on one side of the plantar, the other side of the injection of the same amount of saline, can cause agonist or The number of FOS-positive neurons in the superficial layers was increased in the dorsal horn of spinal cord injected into formalin. The above results suggest that SP has a pain-inducing effect on the periphery that participates in mediating the transmission of nociceptive information to the spinal cord, at least in part, through its binding to SP receptors.