论文部分内容阅读
目的研究干细胞转录因子Oct4在人食管鳞癌中的表达,探讨其在食管鳞癌中发病机制中的作用。方法通过免疫组化及反转录PCR(reverse transcriptase-polymerase chain reaction,RT-PCR)方法检测干细胞转录因子Oct4、Sox2和Nanog在食管鳞癌、良性食管粘膜组织芯片及食管癌肿瘤球中的表达,通过干细胞培养基培养食管癌肿瘤球,检测Oct4在肿瘤球及肿瘤球分化后的细胞表达。结果 Oct4蛋白在绝大多数的食管鳞癌中均表达(45/55,81.8%),但不表达在良性食管粘膜中(0/26,0.0%)。Oct4和Sox2也检测到(分别是42/55,76.4%;39/55,70.9%)。15份新鲜食管癌标本通过干细胞培养基培养均形成了肿瘤球,检测后均表达Oct4蛋白,对比肿瘤球分化后细胞,肿瘤球表达更高水平的Oct4、Nanog和Sox2 mRNA,也表现出更高的增殖潜能。结论 Oct4在食管癌中的高表达提示Oct4在食管癌的发病机制中扮演着重要角色,是食管鳞癌治疗的一个潜在靶点;同时是一个分辨食管鳞癌及良性食管粘膜的方法。
Objective To study the expression of stem cell transcription factor Oct4 in human esophageal squamous cell carcinoma and to explore its role in the pathogenesis of esophageal squamous cell carcinoma. Methods The expressions of Oct4, Sox2 and Nanog were detected by immunohistochemistry and reverse transcriptase polymerase chain reaction (RT-PCR) in esophageal squamous cell carcinoma, benign esophageal mucosa and esophageal cancer The esophageal cancer spheroids were cultured in stem cell culture medium and the expression of Oct4 in the tumor spheres and tumor spheroids was detected. Results Oct4 protein was expressed in most esophageal squamous cell carcinomas (45/55, 81.8%) but not in benign esophageal mucosa (0/26, 0.0%). Oct4 and Sox2 were also detected (42/55, 76.4%; 39/55, 70.9%, respectively). Fifteen samples of fresh esophageal cancer were cultured in the stem cell culture medium to form tumor spheres. After the test, all the cells expressed Oct4 protein. Compared with the tumor spheroids, the tumor cells expressed higher levels of Oct4, Nanog and Sox2 mRNA, The proliferation potential. Conclusions The high expression of Oct4 in esophageal carcinoma suggests that Oct4 plays an important role in the pathogenesis of esophageal carcinoma and is a potential target for the treatment of esophageal squamous cell carcinoma. It is also a method of distinguishing esophageal squamous cell carcinoma from benign esophageal mucosa.