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目的探讨黄河下游济南市段引黄水源水中有机提取物对小鼠的氧化损伤作用。方法于2012年12月采集黄河下游济南市段引黄水源水。将50只昆明健康小鼠随机分为5组,分别为溶剂对照(DMSO)组和2、4、10 L/ml有机提取物暴露组,并设阳性对照(50mg/kg环磷酰胺)组,每组10只,雌雄各半。采用灌胃方式进行染毒,每天1次,连续染毒5 d。测定小鼠血清丙二醛(MDA)、谷胱甘肽(GSH)的含量和超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)的活力。结果与溶剂对照组比较,各剂量有机提取物染毒组雄性小鼠血清MDA、GSH的含量和GSH-Px的活力均升高,SOD活力均下降,差异有统计学意义(P<0.05)。雌性小鼠结果显示,与溶剂对照组比较,2L/ml组血清GSH升高,4、10 L/ml组降低;各染毒组GSH-Px的活力升高;2、4 L/ml组SOD的活力下降,差异均有统计学意义(P<0.05);而各染毒组MDA的含量均无明显变化。结论黄河济南市段引黄水源水中有机提取物对小鼠具有潜在氧化损伤作用。
Objective To investigate the oxidative damage of organic extracts of water from the Yellow River source in Jinan City in the lower Yellow River. Methods In December 2012, the Yellow River source water from the Jinan section of the lower Yellow River was collected. Fifty healthy Kunming mice were randomly divided into five groups: DMSO group and 2,4,10 L / ml organic extract exposure group. A positive control group (50 mg / kg cyclophosphamide) Each group of 10, male and female half. Gavage by way of exposure, 1 times a day, continuous exposure to 5 d. The content of malondialdehyde (MDA) and glutathione (GSH) and the activity of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in mice were determined. Results Compared with the solvent control group, the content of MDA and GSH and the activity of GSH-Px in the male mice exposed to different concentrations of organic extract increased, and the activity of SOD decreased. The difference was statistically significant (P <0.05). The results of female mice showed that serum GSH in 2L / ml group was higher than that in solvent control group, but decreased in 4,10 L / ml group; the activity of GSH-Px in each group was increased; (P <0.05). However, there was no significant change in the content of MDA in all exposed groups. Conclusion The organic extracts of water from the Yellow River in Ji’nan City of the Yellow River have potential oxidative damage effects on mice.