β-地中海贫血产前诊断的新方法——双标记免疫荧光技术

• 作 者: 邱长春
• 出 处: 国外医学(分子生物学分册)
• 发表日期: 1984年05期
• 关键词: β-地中海贫血 同位素掺入法 产前诊断 双标记 胎儿红细胞 胎儿血 产前诊断方法 合成速度 免疫荧光技术 正常胎儿

β-地中海贫血(以下简称β-地贫)是一种遗传性疾病,表现为血红蛋白β链合成减少或完全缺乏.正常胎儿从4～6个月(second trimester)就有一定程度的β链合成,而β-地贫胎儿此时期的β链合成则明显减少或根本不合成.因此,检测胎儿血中β链的有无或多少,就可以诊断β链合成的失调程度.目前,β-地贫的产前诊断方法有两种,第一是用同位素掺入法测定胎儿红细胞中β、γ和α的相对合成速度.该法虽有一定准确性,但有以下缺点:

β-Thalassemia (β-thalassemia) is a hereditary disease characterized by a decrease or complete lack of hemoglobin β-chain synthesis, and normal fetuses have some degree of β-strand synthesis from the second to the second trimester , Whileβ-thalassemia in this period ofβ-strand synthesis decreased significantly or not.So, to detect the presence or absence ofβ-chain in fetal blood, you can diagnose the degree ofβ-chain synthesis disorders.At present, β- Prenatal diagnosis of poor There are two ways, the first is the use of isotope incorporation determination of fetal erythrocyte β, γ and α relative speed of synthesis. Although the method has some accuracy, but has the following disadvantages: