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非接触电导检测法已经成为毛细管电泳芯片中一种通用的物质分离检测方法.本文提出了一种微型化的电容耦合非接触电导分析系统,采用新颖的三明治电极结构减小了电极之间的寄生电容.这种电极结构能够有效地减小电极的长度,克服了非接触电导检测中电极易于折断的缺点.当采用电极宽度1,mm、电极间距1,mm、MES/His缓冲溶液浓度为20,mmol/L和激励电压20,Vpp、90,kHz的条件时,检测器具有最好的分离效果.在最佳的分离条件下,实现了1mmol/L的K+和Mg2+混合无机阳离子的分离检测.
Contactless conductivity detection has become a common method of material separation detection in capillary electrophoresis chip.In this paper, a miniaturized capacitively coupled non-contact conductivity analysis system is proposed, which uses a novel sandwich electrode structure to reduce the parasitic between the electrodes This electrode structure can effectively reduce the length of the electrode and overcome the shortcoming that the electrode is easily broken in the non-contact conductivity detection. When the electrode width is 1 mm, the electrode spacing is 1 mm, and the concentration of the MES / His buffer solution is 20 , mmol / L and excitation voltage of 20, Vpp, 90, kHz, the detector has the best separation effect.Under the optimal separation conditions, the separation and detection of 1mmol / L mixed inorganic cation of K + and Mg2 + .