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采用超临界CO2萃取霉酚酸,通过调节分离釜Ⅰ的压力和温度,增加萃取物的解析选择性,提高分离釜Ⅰ中解析物的霉酚酸纯度.采用2因素5水平的星点设计试验,考察了分离釜Ⅰ的压力和温度对萃取物的霉酚酸含量(CMPA)和霉酚酸萃取回收率(RR)的影响;响应面分析显示,RR与分离釜Ⅰ的压力和温度符合二次模型,压力的一次项和二次项均对RR有极显著影响,温度的一次项也对RR有较显著影响;而CMPA与分离釜Ⅰ的压力和温度符合线性方程,压力对CMPA有极显著影响.根据拟合方程预测分离釜Ⅰ的最佳压力和温度,综合考虑RR和CMPA,确定为14.69 MPa和69.99℃.验证实验中设定分离釜Ⅰ的压力为14.5 MPa、温度为70℃,RR为(91.57±1.31)%,CMPA为(77.50±1.03)%,与预测值90.96%和76.49%接近.
The supercritical CO2 extraction of mycophenolic acid, by adjusting the pressure and temperature of the separation kettle Ⅰ, increase the analytical selectivity of the extract to increase the mycophenolic acid purity of the analyte in the separation kettle I. The design of the two-factor-five , The effects of pressure and temperature of separation kettle Ⅰ on the mycophenolic acid (CMPA) and mycophenolic acid extraction recovery (RR) of extracts were investigated. The response surface analysis showed that the pressure and temperature of RR and separation kettle Ⅰ were in accordance with two In the second model, the first term and the second term of pressure both have a significant effect on RR. The first term of temperature also has a significant effect on RR. However, the pressure and temperature of CMPA and separation kettle Ⅰ are in line with the linear equation. And the optimum pressure and temperature of reactor I were predicted according to the fitting equation, taking the RR and CMPA into consideration, the values were 14.69 MPa and 69.99 ° C. The pressure of the reactor I set in the verification experiment was 14.5 MPa and the temperature was 70 ° C. , RR (91.57 ± 1.31)% and CMPA (77.50 ± 1.03)%, which were close to the predicted values of 90.96% and 76.49%.