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氯化高汞是一种典型的肾毒性毒物,肾脏是它的主要靶器官。氯化高汞主要损害肾近曲小管上皮细胞,可以引起中毒性肾病。为了监测各种原因引起的肾脏损害,人们探索应用了多种指标,其中尿液酶活性的改变作为一种无损伤试验的测定指标,对诊断肾脏损害有一定的价值。本实验用氯化高汞造成大鼠急性肾小管损害,测尿液中酶的活性,观察不同时相,不同剂量对大鼠尿酶活性的影响。材料和方法 1.动物:雌雄大鼠(180—220g),由本院动物房提供。 2.试剂,氯化高汞系上海试剂四厂出品,分析纯,含量不低于99.5％。 3.尿蛋白测定方法:Lowry法。 4.尿肌酐测定方法:苦味酸酯法。
High chloride mercury chloride is a typical toxic nephrotoxicity, the kidney is its main target organ. High chloride main damage to renal proximal tubule epithelial cells, can cause toxic nephropathy. In order to monitor various causes of kidney damage, people explore the use of a variety of indicators, including changes in urinary enzyme activity as a non-invasive test indicators of the diagnosis of kidney damage has a certain value. In this experiment, high chloride mercury chloride caused acute tubular damage in rats, urine enzyme activity was measured to observe the different time and different doses of rat urease activity. Materials and Methods 1. Animals: Male and female rats (180-220g), provided by our hospital animal room. 2. Reagents, chlorinated high-mercury Shanghai Reagent Factory 4 produced, analytical grade, content of not less than 99.5%. 3. Urine protein determination method: Lowry method. Urine creatinine determination method: picrate method.