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背景:丙溴磷为有机磷酸酯类农药杀虫剂,以往对其毒性的研究主要集中在胆碱酯酶的抑制上,而丙溴磷对脂质过氧化及肝功能影响的研究尚较少。目的:探讨丙溴磷对肝组织脂质过氧化及肝功能的影响。设计:完全随机对照的实验研究。地点、材料和干预:本实验在济宁医学院职业卫生与环境医学研究所毒理研究室完成。实验选择3.5月龄的健康家兔42只,雌雄各半。采用随机数字表法将家兔分为高剂量组犤染毒剂量为0.08g/(kg·d)犦、低剂量组犤染毒剂量为0.02g/(kg·d)犦、丙酮对照组3个组,高、低剂量组每组12只,丙酮对照组18只(给予1mL/d丙酮)。测定染毒前、染毒后5,10d家兔血清谷草转氨酶(glutamicoxalacetictransaminaseenzyme,GOT),谷丙转氨酶(glutamic-pyruvictransaminaseenzyme,GPT)活力;分别对染毒前取对照组6只白兔、染毒后5d在3组各取6只家兔、染毒后10d取各组剩余的白兔,进行肝组织中谷胱甘肽过氧化物酶(glutathioneperoxidase,GSH-Px)、超氧化物歧化酶(superoxidedismutase,SOD)的测定。主要观察指标:各组家兔肝组织脂质过氧化指标及肝功能指标。结果:高、低剂量组白兔染毒后5,10d的GSH-Px,GPT,GOT,SOD活力均显著高于丙酮对照组染毒前和处于相同染毒时间的丙酮对照组(t=2.746～10.087,P<0.01);高剂量组染毒后
BACKGROUND: Profenofos is an organophosphate pesticide insecticide. In the past, the studies on its toxicity mainly focused on the inhibition of cholinesterase, while the effects of profenofos on lipid peroxidation and liver function were still few . Objective: To investigate the effects of profenofos on the liver lipid peroxidation and liver function. Design: A fully randomized controlled experimental study. Location, Materials and Interventions: This experiment was performed at the Toxicology Laboratory, Institute of Occupational Health and Environmental Medicine, Jining Medical College. Experimental selection of 3.5-month-old healthy rabbits 42, male and female. The rabbits were randomly divided into high dose group (0.08g / (kg · d) 犦) and low dose group (0.02g / (kg · d) 犦). Acetone group 3 One group, 12 rats in each of high and low dose groups, and 18 rats in acetone control group (given 1 mL / d acetone). The activities of glutamic oxalate transaminase (GOT) and glutamic-pyruvic transaminase (GPT) in rabbits were determined before and 5 and 10 days after exposure. Five rabbits in each group were sacrificed on the 5th day, and the remaining rabbits in each group were sacrificed 10 days after the injection to observe the effects of glutathioneperoxidase (GSH-Px), superoxide dismutase SOD) determination. MAIN OUTCOME MEASURES: Lipid peroxidation index and liver function index of liver tissue of rabbits in each group. Results: The activities of GSH-Px, GPT, GOT and SOD in the high and low dose groups were significantly higher than those in the acetone control group and the acetone exposure group at the same time (t = 2.746 ~ 10.087, P <0.01); high-dose group after exposure