论文部分内容阅读
目的：观察针对ＨＢＶ Ｃ 区双位点核酶对２ ．２ ．１５ 细胞中ＨＢｅＡｇ 表达的抑制作用。方法：利用亚克隆技术，从质粒ｐ ＧＥＭ － Ｒｚ１２３ 切下ＥｃｏＲ Ｉ－ Ｂａｍ Ｈ Ｉ 片段克隆于真核表达质粒ｐＢＢＳ２１２ 中，将该质粒转染２ ．２ ．１５ 细胞，用ＥＬＩＳＡ 及免疫组化方法观察该核酶对ＨＢｅＡｇ 合成的抑制作用。结果：细胞中表达出针对ＨＢＶ Ｃ 区核酶，该核酶对ＨＢｅＡｇ 的抑制率达４８ ．６ ％ 。结论：该双位点核酶可能通过针对ＨＢＶ Ｃ 区基因的剪切作用，阻断Ｃ 区基因的表达
Objective: To observe the double-site ribozyme against HBV C region 2. 2. 15 cells in the inhibition of HBeAg expression. Methods: The EcoR I - Bam H I fragment was excised from the plasmid p GEM - Rz123 and subcloned into eukaryotic expression plasmid pBBS212 using subcloning technique. 2. 15 cells. The inhibitory effect of this ribozyme on HBeAg synthesis was observed by ELISA and immunohistochemistry. Results: The cells expressed HBV C region ribozyme, which inhibited the rate of HBeAg to 48. 6%. Conclusion: The double-site ribozyme may block the expression of C region gene by cutting against the gene of HBV C region