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目的 :探讨前 S1抗原 (Pre- S1)在乙肝诊断中的作用。方法 :用 EL ISA检测 15 3例急、慢性乙肝、乙肝病毒携带者的Pre- S1、抗 HBCIg M、HBVM,并进行比较 ;用荧光定量聚合酶链反应 (FQ- PCR)检测其中 10 6例的 HBVDNA,并以 HBVDNA为金标准 ,对该 10 6例的 Pre- S1及 HBe Ag进行诊断性试验评价。结果 :在急性乙肝中 Pre- S1与抗 HBCIg M的差异有显著性 (P<0 .0 5 ) ;Pre- S1的敏感度 (se)为 94 .2 % ,特异性 (sp)为 90 .7% ,准确度 (accuracy)为 92 .5 % ,高于 HBe Ag的 se 84 .2 % ,sp6 7.6 % ,accuracy 73.6 %。而误诊率α为 0 .0 93,漏诊率β为 0 .0 5 8,低于 HBe Ag的α 0 .32 4 ,β 0 .15 8。结论 :Pre- S1快速 ,准确 ,简便 ,经济 ,可作为乙肝早期诊断及传染性的指标
Objective: To investigate the role of Pre-S1 in the diagnosis of hepatitis B. Methods: ELSA was used to detect Pre-S1, anti-HBCIg M and HBVM in 15 3 acute and chronic hepatitis B and HBV carriers. The levels of Pre-S1, anti-HBcIg M and HBVM were detected by ELISA and 10 6 cases were detected by FQ-PCR Of HBVDNA, and HBVDNA as the gold standard, the 106 cases of Pre-S1 and HBe Ag diagnostic test evaluation. Results: The difference of Pre-S1 and anti-HBCIg M in acute hepatitis B was significant (P <0.05); the sensitivity and the specificity of Pre-S1 were 94.2% and 90 respectively. 7%, accuracy was 92.5%, se 84.2% higher than HBe Ag, sp6 7.6%, accuracy 73.6%. The misdiagnosis rate α was 0.093, the misdiagnosis rate β was 0.058, lower than α 0 .32 4 and β 0. 15 8 of HBe Ag. Conclusion: Pre-S1 is rapid, accurate, simple and economical, which can be used as an indicator of early diagnosis and contagion of hepatitis B