论文部分内容阅读
目的 研究严重急性呼吸综合征 (SARS)患者T淋巴细胞改变特点 ,探讨其发病机制 ,提高SARS的早期诊断水平。方法 收集本院急诊留观的 30例确诊为SARS患者和 5 6名健康献血员的抗凝血 ,用特异性荧光抗体标记 ,通过流式细胞仪检测其CD+ 4和CD+ 8的T淋巴细胞亚群变化 ,对其中 2例恢复后的SARS患者复查CD+ 4和CD+ 8的T淋巴细胞亚群 ,并与人类免疫缺陷病毒 (HIV)、巨细胞病毒 (CMV)和EB病毒 (EBV)感染患者的T淋巴细胞亚群改变进行比较。结果 与正常人相比 ,SARS患者的CD+ 4T淋巴细胞 (简称“T4 细胞”)和CD+ 8T淋巴细胞 (简称“T8细胞”)数量均显著减少 [(2 84± 187)个 /mm3和 (2 88± 16 7)个 /mm3 ],所有SARS患者的T4 细胞计数均低于正常 (最低仅为 49个 /mm3 ) ,恢复期 2例SARS患者的T4 和T8细胞恢复正常 ;与HIV、CMV、EBV感染者相比 ,SARS患者的T4 细胞和T8细胞数量也显著减少 ,而T8细胞数量的减少则更为严重 ;与正常人相比 ,HIV感染者T4 细胞数量也显著减少 ,但HIV、CMV、EBV感染者的T8细胞数量均显著增加。结论 SARS患者的T淋巴细胞数量显著降低 ,但可逆转 ;T4 和T8细胞的检测有助于早期诊断SARS患者。
Objective To study the characteristics of T lymphocyte changes in patients with severe acute respiratory syndrome (SARS) and to explore its pathogenesis and improve the early diagnosis of SARS. Methods Anticoagulant blood was collected from 30 confirmed SARS patients and 56 healthy blood donors in our emergency department and labeled with specific fluorescent antibody. The T lymphocyte subsets of CD + 4 and CD + 8 were detected by flow cytometry The T lymphocyte subsets of CD + 4 and CD + 8 were retrospectively examined in two of the recovered SARS patients and were compared with those of patients infected with human immunodeficiency virus (HIV), cytomegalovirus (CMV) and Epstein-Barr virus (EBV) T lymphocyte subsets changes were compared. Results The numbers of CD + 4T lymphocytes (“T4” cells) and CD + 8 T lymphocytes (“T8 cells”) in patients with SARS were significantly lower than those in healthy controls [(2 84 ± 187) cells / The number of T4 cells in all SARS patients was lower than normal (the lowest was only 49 / mm3). T4 and T8 cells in two SARS patients returned to normal after recovery; The number of T4 cells and T8 cells in patients with SARS was also significantly reduced compared with EBV-infected patients, but the number of T8 cells was even more severely reduced. Compared with normal controls, the number of T4 cells in HIV-infected patients was also significantly reduced. However, HIV, CMV , EBV infected T8 cells were significantly increased. Conclusion The number of T lymphocytes in patients with SARS is significantly reduced but reversible. The detection of T4 and T8 cells is helpful for the early diagnosis of patients with SARS.