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目的 研究HBVD基因型不同毒株全基因进化关系。方法 用聚合酶链式反应(PCR)、克隆及核酸序列测定的方法 ,测定了 1例中国人慢性无症状携带者感染的乙型肝炎病毒D基因型全基因序列。此D基因型毒株全基因序列GenBankAccession为AF2 80 817,将GenBank中已发表的HBVD基因型 30株的全序列进行了系统进化树分析。结果 中国株HBVD基因型与源于瑞典 (Swe den)的 4株HBVD基因型全基因的进化距离最近 ;地中海地区及欧洲国家系HBVD基因型分布的优势地域 ,亚洲并非HBVD基因型分布的优势地域。结论 HBVD基因型病毒株传入来源、迁移的方向不同以及病毒株在具有不同遗传和免疫特质的宿主中的长期选择是形成HBVD基因型病毒进化差异的原因
Objective To study the evolutionary relationships among different genotypes of HBVD genotypes. Methods The complete genomic sequence of hepatitis B virus D genotype in 1 Chinese chronic asymptomatic carrier was determined by polymerase chain reaction (PCR), cloning and nucleic acid sequencing. The GenBank Accession of this D genotype strain was AF2 80 817, and the complete sequence of the 30 published HBVD genotypes in GenBank was analyzed by phylogenetic tree analysis. Results The evolutionary distance between Chinese HBVD genotypes and four strains of HBVD genotypes originating from Sweden was the closest. In the Mediterranean region and European countries, the genotype distribution of HBVD was predominant, while Asia was not the predominant region of HBVD genotype distribution . Conclusion The long-term selection of HBVD genotypes for introduction, migration, and long-term selection of hosts in hosts with different genetic and immunological traits is responsible for the evolutionary divergence of the HBVD genotype virus