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采用两步外植体法,即以百合鳞片叶为初始外植体,以从初始外植体上长出的芽为次级外植体,成功建立了麝香百合的高频离体再生系统。对不同的BA浓度及次级外植体的不同部位对再生效果的影响,以及组培苗移栽前低温处理的影响进行了研究。结果表明:不同部位的次级外植体中,以短缩茎切片出芽快、整齐、芽数多且粗壮;以MS附加1.0 mg L-1 BA和0.1 mg L-1 NAA的培养基最适于麝香百合的分化。一个中等大小已脱春化的鳞茎通过两步外植体法能扩繁出54 000株左右的新植株,从鳞片叶开始至开花仅需8个月,而且4!低温处理对开花期的影响不大。
A two-step explant method was used, in which Lily scale leaf was used as initial explant and the shoots grown from initial explants as secondary explants, the high-frequency ex vivo regeneration system of musk lily was successfully established. The effects of different BA concentrations and different parts of secondary explants on the regeneration effect and the effect of low temperature treatment before transplanting of tissue culture seedlings were studied. The results showed that the shortened stems of the different explants had faster, more neat and more sprouting buds than those of the other explants. MS medium supplemented with 1.0 mg L-1 BA and 0.1 mg L-1 NAA was the best Differentiation of musk lily. A medium-sized, pro-vernalized bulblet can propagate about 54,000 new plants by a two-step explant method, only 8 months from the start of scaly leaf to flowering, and the effect of 4! Low temperature treatment on flowering Not big.