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在溶液聚合体系中,采用“接出”法(“graft from”method)将功能单体4-乙烯基吡啶(4VP)接枝聚合于微米级硅胶表面,制得了功能接枝微粒P4VP/SiO2,采用本课题组建立的新型分子表面印迹技术,以天冬氨酸(Asp)的一种对映体L-Asp为模板分子,二溴己烷为交联剂,对接枝在硅胶表面的大分子P4VP实施了分子印迹,制备了L-Asp分子表面印迹材料MIP-P4VP/SiO2.以天冬氨酸另一种对映体D-Asp为对比物,采用静态与动态两种方法深入研究了MIP-P4VP/SiO2对L-Asp分子的识别性能,重点探索了采用MIP-P4VP/SiO2对天冬氨酸两种对映体进行拆分的可行性.实验结果表明,印迹材料MIP-P4VP/SiO2对模板分子L-Asp具有良好的识别选择性与结合亲和性,相对于D-Asp,识别选择性系数为3.85,对天冬氨酸的外消旋体具有良好的拆分性能.印迹材料MIP-P4VP/SiO2也具有良好的解吸性能,以稀NaOH水溶液作为洗脱液,12个床体积内解吸率即可达到98.52%.
In the solution polymerization system, the functional monomer 4-vinylpyridine (4VP) was graft polymerized on the surface of micron-sized silica gel by the “graft from” method to obtain the functional grafted particles P4VP / SiO2, using the new molecular surface imprinting technology established by our group, using L-Asp, an enantiomer of Asp, as template molecule and dibromohexane as crosslinking agent, The molecular imprinting material MIP-P4VP / SiO2 on the surface of L-Asp was prepared by molecular imprinting on the P4VP of the surface of the silica gel. The other enantiomer D-Asp of aspartic acid was used as the control. Two static and dynamic Methods The recognition properties of L-Asp molecules by MIP-P4VP / SiO2 were studied in detail, and the feasibility of using MIP-P4VP / SiO2 to separate two enantiomers of aspartic acid was also discussed. The experimental results show that the imprinting materials MIP-P4VP / SiO2 has a good recognition selectivity and binding affinity for the template molecule L-Asp, with a recognition selectivity coefficient of 3.85 relative to D-Asp and a good disassembly of aspartic acid racemate The performance of imprinting material MIP-P4VP / SiO2 also has a good desorption performance to dilute aqueous solution of NaOH as eluent, 12 bed volume desorption rate that It reached 98.52%.