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目的分析一例输入性三日疟病例的实验室检测确诊病例。方法对病例的流行病学病史作回顾分析,采集血样作血涂片镜检和疟疾快速诊断(RDT),由浙江省疾病预防控制中心通过巢式PCR检测确诊。结果该病例在本次就诊前一个月,刚从尼日利亚疟疾流行区务工返家,在国外务工期间曾有“疟疾”发病史,回国前后发病间隔2周左右。本次就诊外周血涂片镜检显示为恶性疟感染,RDT结果排除恶性疟感染,巢式PCR检测扩增出三日疟原虫条带后确诊为三日疟。结论疟疾实验室诊断需要综合判断,血涂片镜检和RDT可初步提示疟疾的类型,巢式PCR检测有助于不同疟种感染的鉴别确诊。
Objective To analyze a laboratory-confirmed case of imported malaria cases. Methods The epidemiological history of the cases was retrospectively analyzed. The blood samples were collected for blood smears microscopy and rapid malaria diagnosis (RDT), which was confirmed by nested PCR test by Zhejiang Provincial Center for Disease Control and Prevention. Results One month prior to this visit, the case had just returned from the malaria-endemic area in Nigeria and had a history of “malaria” during the overseas work. The incidence was about two weeks after returning to China. The treatment of peripheral blood smear microscopy showed a falciparum malaria infection, RDT results exclude the infection of falciparum malaria, nested PCR amplification of malaria parasite was amplified three days after the diagnosis of malaria on the 3rd. Conclusion The malaria laboratory diagnosis needs comprehensive judgment. Blood smear microscopy and RDT may initially indicate the type of malaria. Nested PCR may be helpful for the differential diagnosis of different malaria infections.