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目的评价结核感染T细胞斑点试验(T-SPOT.TB)对诊断结核的应用价值。方法对临床确诊的74例肺结核、17例肺外结核和88例健康体检者,进行外周血T-SPOT.TB、皮肤结核菌素试验(PPD)和萋-尼抗酸染色(AFB)的检测,并比较3种方法的诊断价值。结果 T-SPOT.TB检测对肺结核和肺外结核的灵敏度分别为91.89%、88.23%;T-SPOT.TB对结核的特异度为93.18%;PPD和AFB对肺结核的抗体灵敏度分别为70.00%、28.38%,对肺外结核的灵敏度分别为:41.18%、35.29%,特异度分别为95.45%、97.73%。T-SPOT.TB的灵敏度与二者相比,差异有统计学意义(P<0.01),特异度与二者相比,差异无统计学意义。结论酶联免疫斑点T-SPOT.TB实验检测结核敏感性强、特异性高,是快速诊断结核感染的有效手段。
Objective To evaluate the diagnostic value of T-SPOT.TB in diagnosis of tuberculosis. Methods T-SPOT.TB, PPD and AFB were detected in 74 cases of pulmonary tuberculosis, 17 cases of extrapulmonary tuberculosis and 88 cases of healthy volunteers. , And compared the diagnostic value of three methods. Results The sensitivity of T-SPOT.TB to tuberculosis and extrapulmonary tuberculosis was 91.89% and 88.23% respectively. The specificity of T-SPOT.TB to tuberculosis was 93.18%. The sensitivity of PPD and AFB to tuberculosis was 70.00% 28.38%. The sensitivity to extrapulmonary tuberculosis was 41.18% and 35.29% respectively, and the specificity was 95.45% and 97.73% respectively. The sensitivity of T-SPOT.TB was significantly different from that of T-SPOT.TB (P <0.01). The specificity of T-SPOT.TB was not significantly different from that of T-SPOT.TB. Conclusion Enzyme-linked immunosorbent assay (T-SPOT.TB) is a sensitive and rapid method for the detection of tuberculosis. It is an effective method for rapid diagnosis of tuberculosis infection.