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目的考察柿叶提取物自微乳化释药系统(SMEDDS)的大鼠在体肠吸收特征。方法采用大鼠在体小肠灌流实验模型,以高效液相色谱法测定灌流液中槲皮素、山柰酚和酚红的浓度,研究了不同药物浓度、不同吸收部位以及不同剂型对柿叶提取物大鼠肠吸收的影响。结果柿叶提取物自微乳化释药系统浓度的改变对提取物中槲皮素和山柰酚的Ka和P均无显著影响;十二指肠和回肠为柿叶提取物自微乳化释药系统的主要吸收部位;柿叶提取物自微乳化释药系统中槲皮素和山柰酚的吸收速率常数Ka和相对吸收百分率P均显著大于溶液组(P<0.05)。结论柿叶提取物在大鼠小肠主要通过被动扩散方式吸收,柿叶提取物自微乳化释药系统全肠吸收效果优于溶液剂。
Objective To investigate the in vivo intestinal absorption characteristics of the self-microemulsifying drug delivery system (SMEDDS) of persimmon leaf extract. Methods The experimental model of small intestine perfusion in rats was established. The concentrations of quercetin, kaempferol and phenol red in perfusate were determined by high performance liquid chromatography (HPLC). The effects of different drug concentration, different absorption sites and different dosage forms on persimmon leaf extraction Intestinal Absorption in Rats. Results The changes of the self-microemulsifying drug release system of persimmon leaf extract had no significant effect on the Ka and P contents of quercetin and kaempferol in the extracts. The self-microemulsifying drug release of persimmon leaf extract in the duodenum and ileum The absorption rate constant and relative absorption percentage of quercetin and kaempferol in the self-microemulsifying drug delivery system of persimmon leaf extract were significantly higher than that of the solution group (P <0.05). Conclusion Persimmon leaf extract is mainly absorbed in the small intestine of rats by passive diffusion. The total intestinal absorption of persimmon leaf extract self-microemulsifying drug delivery system is better than that of solution.