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目的探索多发性骨髓瘤（ＭＭ）肿瘤克隆性前体细胞作为ＭＭ治疗学上的新的靶细胞的可能性。方法以全反式维甲酸（ＡＴＲＡ）和体外培养的肿瘤克隆性前体细胞为模型，联合应用台盼蓝拒染试验、瘤细胞集落形成试验、光镜下形态学观察、免疫细胞化学染色、扫描电镜技术等方法研究与分析ＡＴＲＡ对肿瘤克隆性前体细胞增殖动力学的影响。结果１３例ＭＭ患者ＰＢＭＮＣｓ样本在体外与ＡＴＲＡ作用１４天后，有６例样本中显示出ＡＴＲＡ对肿瘤克隆性前体细胞有不同程度的抑制作用，有效率为４６．２％。经过ＡＴＲＡ处理过的细胞样本，未见明显的细胞毒作用，培养物中以粒－单细胞集落生长为主，而相应对照组则以瘤细胞集落生长为主。结论ＡＴＲＡ在体外培养系中能明显抑制ＭＭ肿瘤克隆性前体细胞的增殖。肿瘤克隆性前体细胞可能是ＭＭ治疗学上新的靶细胞。
Objective To explore the possibility of multiple myeloma (MM) tumor clonal precursor cells as therapeutic target for new MM cells. METHODS: All-trans retinoic acid (ATRA) and tumor clonal precursor cells cultured in vitro were used as a model, combined with trypan blue exclusion test, tumor cell colony formation assay, light microscope observation, immunocytochemistry staining, Scanning electron microscopy and other methods were used to study and analyze the effects of ATRA on the proliferation kinetics of tumor clonal precursor cells. Results After 14 days of ABM in vitro and PBMNCs samples from 13 cases of MM patients, ATRA showed different degrees of inhibition on clonal precursor cells in 6 cases, and the effective rate was 46.2%. After ATRA-treated cell samples, no obvious cytotoxicity was observed. In the cultures, granule-single cell colony growth was dominant, while in the corresponding control group, tumor cell colony growth was dominant. Conclusion ATRA can significantly inhibit the proliferation of MM tumor clonal precursor cells in vitro. The tumor clonal precursor cells may be new target cells for MM therapeutics.