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EB病毒不能在其转化的淋巴细胞中引起大量溶解性感染,因此采用常规的方法分离EB病毒DNA比较困难。以往的分离方法需要从大量的部分感受态的B-淋巴细胞培养物中增殖病毒粒子,故分离到的纯EB DNA量很少(每10~(10)个细胞中只获得1
Epstein-Barr virus does not cause a large number of lytic infections in its transformed lymphocytes. Therefore, it is difficult to isolate Epstein-Barr virus DNA by conventional methods. Previous isolation methods required the propagation of virions in a large number of partially competent B-lymphocyte cultures, so that the amount of pure EB DNA isolated was small (only 1 was obtained for every 10 to 10 cells