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目的:分析卷柏(Selaginella tamariscina)中糖类化学成分,观察其对中国仓鼠肺成纤维细胞(V79)和BALB/c小鼠的辐射防护作用,为探讨卷柏抗辐射作用机制提供参考依据。方法:薄层色谱法分析卷柏中糖类化学成分;V79细胞经木糖、果糖、阿拉伯糖处理24h后接受60Coγ射线照射,分别观察细胞活力、克隆形成率和细胞周期;BALB/c小鼠接受6Gy60Coγ射线照射前1周给予木糖,观察受照射小鼠的30d存活率。结果:卷柏中含有葡萄糖、木糖、果糖、阿拉伯糖、海藻糖等;其中木糖、果糖、阿拉伯糖在20～200μg·mL-1浓度范围内可剂量依赖地促进照射后V79细胞增殖,在200μg·mL-1时可提高照射后V79细胞克隆形成率,增加S期细胞比例,缓解G2/M期阻滞,修正紊乱的细胞周期;照射前分别给予木糖100,500mg·kg-1可剂量依赖地提高受照射小鼠存活率达20%,30%。结论:卷柏中的单糖类成分可提高受照射小鼠的30d存活率,其机制可能是通过调节受照射后紊乱的细胞周期而发挥辐射防护作用。
OBJECTIVE: To analyze the chemical constituents of sugar in Selaginella tamariscina and observe its radiation protective effects on Chinese hamster lung fibroblasts (V79) and BALB/c mice to provide reference for the study of the anti-radiation mechanism of Selaginella. METHODS: TLC was used to analyze the chemical constituents of sugars in Selaginella; V79 cells were exposed to 60Co γ-rays after 24 h treatment with xylose, fructose and arabinose. Cell viability, colony formation rate and cell cycle were observed. BALB/c mice were observed. Xylose was given 1 week before 6Gy60Co γ-ray irradiation, and the 30-day survival rate of irradiated mice was observed. Results: Selaginella contains glucose, xylose, fructose, arabinose, and trehalose. Xylose, fructose and arabinose can increase the proliferation of V79 cells in dose-dependent manner within the range of 20-200 μg·mL-1. At 200 μg·mL-1, the colony forming rate of V79 cells after irradiation was increased, the proportion of cells in S phase was increased, the blockage of G2/M phase was alleviated, and the disordered cell cycle was corrected; Xylose 100, 500 mg·kg-1 was given before irradiation. Dose-dependently increased the survival rate of irradiated mice by 20% and 30%. Conclusion: The monosaccharides in Selaginella communis can increase the survival rate of irradiated mice for 30 days. The mechanism may be that it plays a role in radiation protection by regulating the cell cycle after irradiation.