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目的:探讨信号转导和转录激活因子-5(Signal transducer and activator of transcripton 5,STAT5)、WW 结构域氧化还原酶基因(WW domain-containing oxidoreductase,WWOX)和 c-myc 蛋白在非小细胞肺癌(non smal cel lung cancer,NSCLC)中的表达及相关性研究.方法:用免疫组化 SP 法检测40例手术切除的 NSCLC组织和20例正常肺组织中 STAT5、WWOX 和 c-myc 的蛋白表达.结果:STAT5和 c-myc 蛋白在 NSCLC 中的表达明显高于正常肺组织,而 WWOX 在 NSCLC 中的表达明显低于正常肺组织(P﹤0.01);STAT5、c-myc 表达水平与 NSCLC 组织学类型有关,在腺癌组织中的表达明显高于鳞癌(P﹤0.01), WWOX 基因的表达与淋巴结转移相关,有淋巴结转移的表达低于无淋巴结结转移(P﹤0.05);STAT5蛋白和 wwox 蛋白在 NSCLC 组织中的表达呈负相关(P﹤0.05),而 STAT5和 c-myc 的表达、WWOX 与 c-myc 的表达无相关性(P >0.05).结论:在 NSCLC 中存在 STAT5和 c-myc 的过度表达以及 WWOX 的失表达,STAT5、c-myc 表达水平与 NSCLC 组织学类型有关,而 WWOX 的表达与淋巴结转移相关.在 NSCLC 的发生机制中,提示 WWOX 可能通过抑制 STATs 系统,促进细胞凋亡,起到抑癌作用.“,”Objective:To investigate the expression of STAT5 ,WWOX and c-myc in non smal cel lung cancer(NSCLC) and their clinical significance. Method: Forty resected tissue samples from patients with NSCLC and twenty normal lung tissues were subjected to immunohistochemical staining(SP) for STAT5 ,WWOX and c-myc. Results: The expression of STAT5 and c-myc is apparently rised and the expression of wwox is apparently lower in NSCLC than these in normal lung tissues, this has a significance (P﹤0.01). The expression of STAT5and c-myc are concerned with NSCLC histological types, in adenocarcinoma was significantly higher than squamous cel carcinoma (P ﹤0.01); WWOX gene expression and whether there is lymph node metastasis , with lymph node metastasis NSCLC loss of WWOX gene expression or express a more reduced(P﹤0.05). There was a negative correlation between the expressions of STAT5 and WWOX.But no relationship was found between STAT5 and c-myc or WWOX and c-myc (P > 0.05). Conclusion: STAT5 and c-myc expression rised in NSCLC was significantly than in normal lung tissue, and similarly, WWOX in NSCLC tissue was low, and its expression in the absence or reduction of tumor invasion may be related. STAT5, c-myc expression are concerned with the NSCLC histological types;WWOX gene expression is concerned with whether the lymph node metastasis, loss or reduction of its expression with tumor aggressiveness. WWOX expression was negatively related to the higher expression of STAT5 in NSCLC. In the generate mechanism of the NSCLC ,it prompt that WWOX might pass to refrain STATs,to advance apoptosis and to restrain the NSCLC.