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将感染6～8周的纯种小鼠(CBA/Ca)处死取肝,采用Wellhausen等(1981)的方法得到肉芽肿细胞悬液,取一部份置塑料管中进行孵育,大多数巨噬细胞(m(?))粘附于管壁而嗜酸粒细胞则大多留在悬液中。用物理方法将m(?)移入另一试管中,分别制成m(?)及嗜酸粒细胞悬液;正常及免疫脾细胞分别取自未感染和感染血吸虫的小鼠。正常鼠血清(NMS)和免疫鼠血清(IMS)分别获自未感染及感染16～20周的小鼠;从首次感染的Sprague-Dawley大鼠收集的新鲜血清(fNRS)用作补体。非特异m(?)通过腹腔注射
The purebred mice (CBA / Ca) infected 6 to 8 weeks were sacrificed and the granuloma suspension was obtained by the method of Wellhausen et al. (1981). Part of the suspension was placed in plastic tubes and incubated. The majority of macrophages Cells (m (?)) Adhere to the wall of the tube while eosinophils mostly remain in the suspension. Physically, m (?) Was transferred to another test tube to form m (?) And eosinophil suspension, respectively; normal and immunized splenocytes were taken from mice that were uninfected and infected with schistosomiasis, respectively. Normal rat serum (NMS) and immunized rat serum (IMS) were obtained from uninfected and infected mice for 16 to 20 weeks respectively; fresh serum (fNRS) collected from naive Sprague-Dawley rats was used as complement. Non-specific m (?) By intraperitoneal injection