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业已证明,经过有丝分裂原刺激的淋巴样细胞,其辐射耐受性比分裂间期的淋巴样细胞高。脐带血造血祖细胞的DNA自发合成率高,且能在体外培养下自发生成集落。因此有必要和有条件去研究其辐射敏感性。实验用健康足月胎儿分娩后5小时内的脐带血。对照为健康成人外周血。把经分离提纯的单个核细胞和加或不加CSF的血沉棕黄层细胞用15Mev的电子感应加速器生成的加速电子分别照射10、100、200、400、600、800、1200、1500拉德(剂量率为200拉德/分)。照射后的细胞用甲基纤维素培养体系检测CFU—GM;
It has been demonstrated that lymphocytes that are stimulated by mitogen are more tolerant of radiation than lymphoid cells in the interphase. Umbilical cord blood hematopoietic progenitor cells DNA spontaneous synthesis rate, and in vitro culture into spontaneous colonies. Therefore, it is necessary and conditional to study its radiation sensitivity. Cord blood test within 5 hours after birth in healthy full-term fetus. Control for healthy adult peripheral blood. Accelerated electrons generated by the isolated purified mononuclear cells and buffy coat cells with or without CSF were irradiated with 15 MeV electron-induced accelerators at 10, 100, 200, 400, 600, 800, 1200, 1500 rad Dose rate of 200 rad / min). The irradiated cells were tested for CFU-GM using a methylcellulose culture system;