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作者用10株东非利什曼原虫株(其中2株为皮肤型,8株为内脏型),于不同的培养基中培养,对其生长情况进行了比较。选用的培养基包括市售的施奈德化果蝇Schneider’s Drosophila培养基、格雷斯氏昆虫组织培养基、Singh’s蚊虫培养基和RPMI 1640培养基。利什曼鞭毛体均于7.5%二甲基亚枫-RPMI 1640中冰冻保存,介冻后立即接种于培养基中。实验用液体培养基加入25ml组织培养瓶中,每瓶加5ml,血液基础培养基1ml放于16×110mm塑料组织培养管中成斜面,接种前加2ml培养液或盐水。
The authors used 10 Leishmania leishmanii strains (2 of which were skin type and 8 were visceral) and cultured in different media to compare their growth. Selected media include the commercially available Schneider’s Drosophila Schizomes, Grace’s Tissue, Singh’s Mosquito and RPMI 1640 media. Leishmania flagellate were cryopreserved in 7.5% DMSO-RPMI 1640 and immediately inoculated into medium after medium freezing. Experiment with liquid medium into 25ml tissue culture flask, add 5ml per bottle, blood basic medium 1ml placed in 16 × 110mm plastic tissue culture tube beveled, add 2ml before inoculation culture medium or saline.