论文部分内容阅读
目的分析接种含麻疹成分疫苗(Measles containing vaccine,MCV)后发热出疹病例(Rash and fever illness,RFI)的麻疹血清学和病毒学检测特征。方法采用酶联免疫吸附试验(ELISA)检测接种MCV后RFI的血清麻疹IgM抗体;用逆转录-聚合酶链反应(Reverse transcript polymerase chain reaction,RT-PCR)对麻疹病毒RNA样本进行检测,扩增麻疹病毒核蛋白(Nucleoprotein,N)基因羧基末端450个核苷酸片段;对扩增产物进行核苷酸序列测定和分析。结果 2010-2013年广东省共收检723例RFI的病原学标本,其中18例为接种MCV后RFI。在18例RFI中17例采集了血清标本,麻疹IgM抗体均阳性;18例病例的病原学样本经过序列测定分析,均属于A基因型,与中国S191疫苗株的核苷酸同源性为99.7%-100%。结论 MCV接种后RFI主要为麻疹疫苗相关病例;需加强RTPCR和核酸测序方法对病例的快速检测。
Objective To analyze the measles serological and virological characteristics of measles containing vaccine (Measles containing vaccine, MCI) after inoculation with Rash and fever illness (RFI). Methods Serum measles IgM antibody against RFI was detected by enzyme-linked immunosorbent assay (ELISA). The RNA samples of measles virus were detected by reverse transcriptase-polymerase chain reaction (RT-PCR) 450 nucleotides of the carboxyl terminus of the nucleoprotein (N) gene of measles virus; the nucleotide sequence of the amplified product was determined and analyzed. Results A total of 723 RFI etiological specimens were collected from Guangdong Province during 2010-2013, of which 18 were RFI after MCV inoculation. Serum samples were collected from 17 RFI patients and measles IgM antibodies were positive. Eighteen cases of pathogenic samples were sequenced and analyzed, all of which belonged to A genotype. The nucleotide homology with China S191 vaccine strain was 99.7 % -100%. Conclusion The RFI after MCV inoculation is mainly related to measles vaccine; RTPCR and nucleic acid sequencing methods need to be strengthened to detect the cases rapidly.